Flavobacterium ginsengisoli sp nov., isolated from soil of a ginseng field

A novel bacterial strain, designated DCY54(T), was isolated from a field cultivated with ginseng in Yongin, Republic of Korea. Cells were Gram-reaction-negative, yellow-pigmented, rod-shaped, non-spore-forming, and strictly aerobic. They were motile by gliding and produced flexirubin-type pigments. Growth occurred optimally at 25-30 degrees C, at pH 5.0-7.0 and in the presence of 0-1% NaCl. The 16S rRNA sequence analysis demonstrated that strain DCY54T was most closely related to Flavobacterium defluvii EM B 117(T) (96.9%). The only isoprenoid quinone of strain DCY 54(T) was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The major cellular fatty acids (>15%) were isoC(15:0), summed feature 3 (comprising C-16:1 omega 7c and/or iso-C-15:0 2-OH) and C-16:0. The DNA G+C content was 33.3 mol%. Phylogenetic inference and phenotypic data supported affiliation of strain DCY54T to the genus Flavobacterium. Several physiological and biochemical tests differentiated strain DCY54(T) from the species of the genus Flavobacterium with validly published names. On the basis of data from a polyphasic study, strain DCY54(T) represents a novel species of the genus Flavobacterium for which the name Flavobacterium ginseng/soli sp. nov. is proposed. The type strain is DCY54T (=KCTC 23318(T)=JCM 17336(T)).