New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification
被引:10
作者:
Mu, Yonglin
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机构:
Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Mu, Yonglin
[1
,2
]
Zeng, Jiawei
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机构:
Dujiangyan Med Ctr, Dujiangyan, Sichuan, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Zeng, Jiawei
[4
]
Chen, Qianqian
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R China
Jiangsu Univ, Inst Life Sci, Zhenjiang, Jiangsu, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Chen, Qianqian
[2
,3
]
Liu, Jia
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Liu, Jia
[2
]
Wang, Lili
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Wang, Lili
[2
]
Yao, Fujia
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Yao, Fujia
[2
]
Cui, Meng
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Cui, Meng
[2
]
He, Zhixiang
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
He, Zhixiang
[2
]
Zhang, Chiyu
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机构:
Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Zhang, Chiyu
[2
]
Xiao, Ming
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Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Xiao, Ming
[1
]
Lan, Ke
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Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R ChinaShanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
Lan, Ke
[2
]
机构:
[1] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
[2] Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, Shanghai, Peoples R China
[3] Jiangsu Univ, Inst Life Sci, Zhenjiang, Jiangsu, Peoples R China
[4] Dujiangyan Med Ctr, Dujiangyan, Sichuan, Peoples R China
Human respiratory syncytial virus (HRSV) is a seasonal respiratory pathogen that causes respiratory infection in children and the elderly. Anew, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid (within 1 h), simultaneous detection of A and B group HRSV. Primers specific for groups A and B were designed to amplify the N and L genes of HRSV, respectively. A fluorescent dye, calcein, was used as an indicator for the endpoint visual detection and/or real-time amplification of HRSV RNA:The detection limit of the new method was 281.17 50% tissue culture infective doses (TCID50)/ml for HRSV A and 1.58 TCID50/ml for HRSV B. To evaluate the validity of this method, a comparison with RT-PCR was performed using 77 nasopharyngeal swabs as samples. Both RT-LAMP and RT-PCR detected HRSV in 38 HRSV samples, yielding a positive rate of 49%. Of the RI-LAMP positive samples, 36 (95%) were also positive by RT-PCR, while two were negative by RT-PCR. Among the 36 RT-LAMP and RT-PCR positive samples, 11 belonged to HRSV group A, while 25 belonged to group B. The results show that the new RI-LAMP is simple, rapid and well suited for HRSV diagnosis, especially in a limited-resource setting. (C) 2014 Elsevier B.V. All rights reserved.
机构:
NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
Inst Salud Carlos III, CIBER Enferrnedades Resp, Madrid 28220, SpainNIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
Collins, Peter L.
;
Melero, Jose A.
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机构:
Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid 28220, Spain
Inst Salud Carlos III, CIBER Enferrnedades Resp, Madrid 28220, SpainNIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
机构:
NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
Inst Salud Carlos III, CIBER Enferrnedades Resp, Madrid 28220, SpainNIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
Collins, Peter L.
;
Melero, Jose A.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid 28220, Spain
Inst Salud Carlos III, CIBER Enferrnedades Resp, Madrid 28220, SpainNIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA