Recent Progress of Microfluidic Techniques for Protein Crystallization and Screening

被引：5

作者：

Zhu Lina ^{[1
]}

Zhu Ying ^{[1
]}

Fang Qun ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Inst Microanalyt Syst, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2014年 / 35卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Microfluidics; Protein crystallization; PDMS chip-based pump and valve; Droplet; Membrane protein; FREE INTERFACE DIFFUSION; LIPIDIC MESOPHASES; MEMBRANE-PROTEINS; NANOLITER PLUGS; PHASE-BEHAVIOR; BIOLOGICAL MACROMOLECULES; STRUCTURAL BIOLOGY; DRUG DISCOVERY; CRYSTAL-GROWTH; SYSTEM;

D O I：

10.7503/cjcu20130691

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

With the advantages of high throughput, low consumption and large-scale integration, microfluidics has proven to be a promising technique for protein crystallization and screening. This article reviews the recent progress of various microfluidic techniques successfully applied in protein crystallization, including PDMS-based valve and pump, droplet, SlipChip and DropLab. In addition, due to the high importance and great challenges of membrane proteins in structural biology, we further introduce the microfluidic techniques for crystallization of membrane proteins.

引用

页码：1 / 11

页数：11

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