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Ultrasensitive immunoassay of Staphylococcus aureus based on colorimetric and fluorescent responses of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole to L-cysteine
被引：20
作者：
Deng, Wenfang
[1
]
Cheng, Chang
[1
]
Yang, Hui
[1
]
Wang, Hui
[1
]
Tan, Yueming
[1
]
Xie, Qingji
[1
]
Ma, Ming
[1
]
Yao, Shouzhuo
[1
]
机构：
[1] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China
来源：
基金：
中国国家自然科学基金;
关键词：
Colorimetric and fluorescent immunoassay;
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole;
L-cysteine;
Pathogenic bacteria;
Staphylococcus aureus;
PATHOGENIC BACTERIA;
ESCHERICHIA-COLI;
CARBON DOTS;
NANOPARTICLES;
APTASENSOR;
PROBE;
ASSAY;
D O I：
10.1016/j.talanta.2019.04.076
中图分类号：
O65 [分析化学];
学科分类号：
070302 ;
081704 ;
摘要：
We report here a sensitive method for immunoassay of Staphylococcus aureus (S. aureus) based on colorimetric and fluorescent responses of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to L-cysteine (Cys). S. aureus cells were separated from samples by immunomagnetic nanoparticles and labeled with Cys-encapsulated liposome (Cys@liposome) immuno-nanocapsules. After mangnetic separation, the Cys@liposome immuno-nanocapsules linked to bacterial cells were destructed by Tween-20, and then the released Cys molecules reacted with NBD-Cl to generate colorimetric and fluorescent signals. Due to the amplification effect of liposome nanocapsules, the high capture efficiency of immunomagnetic nanoparticles, and the high responses of NBD-Cl to Cys, the detection limits for colorimetric and fluorescent detection of S. aureus were as low as 10 and 1 CFU mL(-)(1), respectively. The proposed method was successfully used to detect S. aureus in milk samples. This work has provided a novel NBD-Cl based colorimetric and fluorescent platform for immunoassay.
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页码：244 / 250
页数：7
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