Structure-function analysis of the anti-proliferative effect of the human guanylate binding protein-1 (GBP-1) in endothelial cells

被引：0
作者：
Töpolt, K [1 ]
Guenzi, E [1 ]
Lubeseder-Martellato, C [1 ]
Jörg, A [1 ]
Naschberger, E [1 ]
Stürzl, M [1 ]
机构：
[1] GSF, Natl Res Ctr Environm & Hlth, Inst Mol Virol, Dept Virus Induced Vasculopathy, Neuherberg, Germany
来源：
22ND MEETING OF THE EUROPEAN SOCIETY FOR MICROCIRCULATION: MICROCIRCULATION AND VASCULAR BIOLOGY | 2002年
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中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Inflammatory cytokines (IC) are potent inhibitors of endothelial cell proliferation. As a specific mediator of the anti-proliferative effect of IC on endothelial cells (EC) we identified the large GTPase human guanylate binding protein-1 (GBP-1). Ectopic expression of GBP-1 in EC inhibited angiogenic growth factor (AGF)-induced proliferation, whereas inhibition of IC-induced GBP-1 expression by GBP-1 antisense RNA abrogated the anti-proliferative effect of IC. GBP-1 selectively inhibited the proliferation of endothelial cells in the absence of apoptosis and without affecting adhesiveness for monocytes, morphogenesis or ERK 1/2 activation. Structure-function-analysis conveyed that the anti-proliferative effect is mediated specifically by the helical domain of GBP-1 and independent of isoprenylation or GTP-hydrolysis.
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页码：215 / 219
页数：5
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