Detection of peroxynitrite anion released from HeLa cells via hyper-cross-linked polymer nanotube modified electrode

Abnormal expression levels of peroxynitrite anion (ONOO-) in human cells have relation to many diseases, especially cancer. Here, we constructed a novel sensing platform for directly detecting ONOO- using hyper-cross-linked polymeric nanotubes (HNTs)-modified glassy carbon electrode. The HNTs nanohybrids were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), FT-IR spectroscopy, and cyclic voltammetry (CV). The prepared HNTs/GCE sensor exhibited outstanding performance in detecting ONOO-, which provided a wide linear range from 2.34 x 10(-8) M to 1.74 x 10(-4) M and a low detection limit of 7.43 nM (S/N = 3). Moreover, it was also used to measure the ONOO- released from HeLa cells induced by stimulus. The designed sensor may provide great potential for expand applications in the matter of cancer treatment and biomedicine.