DNA maintenance methylation enzyme Dnmt1 in satellite cells is essential for muscle regeneration

被引：8
|
作者：
Iio, Hiroyuki [1 ,2 ]
Kikugawa, Tadahiko [1 ]
Sawada, Yuichiro [1 ,2 ]
Sakai, Hiroshi [2 ,3 ]
Yoshida, Shuhei [3 ]
Yanagihara, Yuta [2 ,4 ]
Ikedo, Aoi [2 ]
Saeki, Noritaka [2 ,4 ]
Fukada, So-ichiro [5 ]
Saika, Takashi [1 ]
Imai, Yuuki [2 ,3 ,4 ]
机构：
[1] Ehime Univ, Grad Sch Med, Dept Urol, Shitsukawa, Toon Ehime 7910295, Japan
[2] Ehime Univ, Div Integrat Pathophysiol, Proteosci Ctr, Shitsukawa, Toon Ehime 7910295, Japan
[3] Ehime Univ, Dept Pathophysiol, Grad Sch Med, Shitsukawa, Toon Ehime 7910295, Japan
[4] Ehime Univ, Div Lab Anim Res, Adv Res Support Ctr, Shitsukawa, Toon Ehime 7910295, Japan
[5] Osaka Univ, Grad Sch Pharmaceut Sci, Project Muscle Stem Cell Biol, Suita, Osaka 5650871, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 534卷
基金：
日本学术振兴会;
关键词：
Dnmt1; DNA methylation; Epigenetics; Skeletal muscle; Muscle satellite cells; Muscle regeneration; STEM-CELLS; HYPERTROPHY; PAX7;
D O I：
10.1016/j.bbrc.2020.11.116
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Epigenetic transcriptional regulation is essential for the differentiation of various types of cells, including skeletal muscle cells. DNA methyltransferase 1 (Dnmt1) is responsible for maintenance of DNA methylation patterns via cell division. Here, we investigated the relationship between Dnmt1 and skeletal muscle regeneration. We found that Dnmt1 is upregulated in muscles during regeneration. To assess the role of Dnmtl in satellite cells during regeneration, we performed conditional knockout (cKO) of Dnmt1 specifically in skeletal muscle satellite cells using Pax7(CreERT2) mice and Dnmt1 flox mice. Muscle weight and the cross-sectional area after injury were significantly lower in Dnmt1 cKO mice than in control mice. RNA sequencing analysis revealed upregulation of genes involved in cell adhesion and apoptosis in satellite cells from cKO mice. Moreover, satellite cells cultured from cKO mice exhibited a reduced number of cells. These results suggest that Dnmt1 is an essential factor for muscle regeneration and is involved in positive regulation of satellite cell number. (C)2020 Elsevier Inc. All rights reserved.
引用
收藏
页码：79 / 85
页数：7
相关论文
共 50 条
  • [1] Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration
    Anderson, Ryan M.
    Bosch, Justin A.
    Goll, Mary G.
    Hesselson, Daniel
    Dong, P. Duc Si
    Shin, Donghun
    Chi, Neil C.
    Shin, Chong Hyun
    Schlegel, Amnon
    Halpern, Marnie
    Stainier, Didier Y. R.
    DEVELOPMENTAL BIOLOGY, 2009, 334 (01) : 213 - 223
  • [2] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development
    Hirasawa, Ryutaro
    Chiba, Hatsune
    Kaneda, Masahiro
    Tajima, Shoji
    Li, En
    Jaenisch, Rudolf
    Sasaki, Hiroyuki
    GENES & DEVELOPMENT, 2008, 22 (12) : 1607 - 1616
  • [3] DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis
    Pathania, Rajneesh
    Ramachandran, Sabarish
    Elangovan, Selvakumar
    Padia, Ravi
    Yang, Pengyi
    Cinghu, Senthilkumar
    Veeranan-Karmegam, Rajalakshmi
    Arjunan, Pachiappan
    Gnana-Prakasam, Jaya P.
    Sadanand, Fulzele
    Pei, Lirong
    Chang, Chang-Sheng
    Choi, Jeong-Hyeon
    Shi, Huidong
    Manicassamy, Santhakumar
    Prasad, Puttur D.
    Sharma, Suash
    Ganapathy, Vadivel
    Jothi, Raja
    Thangaraju, Muthusamy
    NATURE COMMUNICATIONS, 2015, 6
  • [4] DNA methylation and the activity pattern of DNA methyltransferase (DNMT1) in endometrial carcinoma
    Romanek-Piva, Katarzyna
    Galczynski, Krzysztof
    Adamiak-Godlewska, Aneta
    Rechberger, Tomasz
    Postawski, Krzysztof
    GINEKOLOGIA POLSKA, 2016, 87 (01) : 6 - 10
  • [5] BAH domains and a histone-like motif in DNA methyltransferase 1 (DNMT1) regulate de novo and maintenance methylation in vivo
    Yarychkivska, Olya
    Shahabuddin, Zoha
    Comfort, Nicole
    Boulard, Mathieu
    Bestor, Timothy H.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2018, 293 (50) : 19466 - 19475
  • [6] Targeting sequence domain: a captain at the helm precisely steering DNMT1 through maintenance methylation?
    Klein, Christopher
    EPIGENOMICS, 2016, 8 (06) : 737 - 740
  • [7] Identification of DNMT1 (DNA methyltransferase 1) hypomorphs in somatic knockouts suggests an essential role for DNMT1 in cell survival
    Egger, Gerda
    Jeong, Shinwu
    Escobar, Sonia G.
    Cortez, Connie C.
    Li, Tony W. H.
    Saito, Yoshimasa
    Yoo, Christine B.
    Jones, Peter A.
    Liang, Gangning
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (38) : 14080 - 14085
  • [8] Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA
    Fatemi, M
    Hermann, A
    Gowher, H
    Jeltsch, A
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (20): : 4981 - 4984
  • [9] More Than DNA Methylation: Does Pleiotropy Drive the Complex Pattern of Evolution of Dnmt1?
    Amukamara, Ashley U.
    Washington, Joshua T.
    Sanchez, Zachary
    McKinney, Elizabeth C.
    Moore, Allen J.
    Schmitz, Robert J.
    Moore, Patricia J.
    FRONTIERS IN ECOLOGY AND EVOLUTION, 2020, 8
  • [10] BRCA1 affects global DNA methylation through regulation of DNMT1
    Shukla, Vivek
    Coumoul, Xavier
    Lahusen, Tyler
    Wang, Rui-Hong
    Xu, Xiaoling
    Vassilopoulos, Athanassios
    Xiao, Cuiying
    Lee, Mi-Hye
    Man, Yan-Gao
    Ouchi, Mutsuko
    Ouchi, Toru
    Deng, Chu-Xia
    CELL RESEARCH, 2010, 20 (11) : 1201 - 1215
12345