DIAGNOSES OF LEPTOSPIROSIS IN ANIMALS

被引:0
作者
Nurlygayanova, Gulnara [1 ]
Belousov, Vasily [1 ]
Varentsova, Alisa [1 ]
Ozheredova, Nadezhda [2 ]
Chernykh, Oleg [3 ]
机构
[1] Fed State Budgetary Inst, Dept Short Res Papers, Cent Sci & Method Vet Lab, Rostov Na Donu, Russia
[2] Stavropol State Agr Univ, Dept Epizootol & Microbiol, Stavropol, Russia
[3] Kuban State Agr Univ Named IT Trubilina, Dept Microbiol Epizootol & Virol, Krasnodar, Russia
来源
INTERNATIONAL TRANSACTION JOURNAL OF ENGINEERING MANAGEMENT & APPLIED SCIENCES & TECHNOLOGIES | 2020年 / 11卷 / 15期
关键词
Dark-Field Microscopy; Microagglutination Reaction; PCR; Bacteriological studies; Infectious jaundice; Microscopic agglutination test (MAT);
D O I
10.14456/ITJEMAST.2020.304
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
There have been presenting a comparative analysis of the results of leptospirosis diagnosis by methods used in the Russian Federation's veterinary laboratories. Using the microagglutination reaction, specific antibodies to leptospira were detecting in 136862 animals. A total of 64686 urine samples was examined with dark-field microscopy. Applying PCR, the leptospira genome is detected in pathological material in 57 cases. The PCR method in the water of open reservoirs cannot detect the pathogenic leptospira genome. In the study of biological material, the leptospira genome was found in 248 cases. To increase the efficiency of diagnosis and reduce the time of diagnosis for leptospirosis, it is advisable to conduct parallel bacteriological studies of the material in combination with PCR analysis. Micro-agglutination reaction and molecular genetic analysis (PCR) are the most inforeaction micro-agglutinative and technologically advanced laboratory diagnostic methods. Disciplinary: Biology, Epizootology, and Microbiology, Bioscience, Veterinary Sciences. (c) 2020 INT TRANS J ENG MANAG SCI TECH.
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页数:9
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