Genetic diversity assessment in greek Medicago truncatula genotypes using microsatellite markers

被引：9
作者：
Akritidis, P. [1 ]
Mylona, P. V. [2 ]
Tsaftaris, A. S. [1 ,3 ]
Polidoros, A. N. [1 ]
机构：
[1] CERTH, Inst Agrobiotechnol, GR-57001 Thermi, Greece
[2] NAGREF, Agr Res Ctr No Greece, GR-57001 Thermi, Greece
[3] AUTh, Dept Genet & Plant Breeding, GR-54006 Thessaloniki, Greece
来源：
BIOLOGIA PLANTARUM | 2009年 / 53卷 / 02期
关键词：
molecular markers; phylogenetic analysis; simple sequence repeats; MODEL LEGUME; GENOME;
D O I：
10.1007/s10535-009-0063-6
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In this study we examined the genetic diversity and geographic scale of genotype distribution within the model legume species Medicago truncatula widely distributed in pasture and marginal agricultural lands in Greece and other Mediterranean countries. Thirty one Medicago truncatula and Medicago littorialis accessions were chosen on the basis of their geographical distributions and studied using 9 polymorphic simple sequence repeats (SSR) markers. The number of alleles per locus varied between 3 and 7. A total of 42 alleles were detected with a mean value of 4.66 alleles per locus. Geographic origin was not related with genotypic similarity among accessions. However, there were instances of close genetic relatedness between accessions from neighboring locations in a geographic compartment. In conclusion, the presented data revealed extensive M. truncatula genotype dispersal in Greece pointing to the significance of preserving local genetic resources in their natural environment.
引用
收藏
页码：343 / 346
页数：4
相关论文
共 15 条
[1]  
Baquerizo-Audiot E, 2001, MOL ECOL NOTES, V1, P1, DOI 10.1046/j.1471-8278.2000.00001.x
[2]   Spatial effects and rare outcrossing events in Medicago truncatula (Fabaceae) [J].
Bonnin, I ;
Ronfort, J ;
Wozniak, F ;
Olivieri, I .
MOLECULAR ECOLOGY, 2001, 10 (06) :1371-1383
[3]   Estimating genome conservation between crop and model legume species [J].
Choi, HK ;
Mun, JH ;
Kim, DJ ;
Zhu, HY ;
Baek, JM ;
Mudge, J ;
Roe, B ;
Ellis, N ;
Doyle, J ;
Kiss, GB ;
Young, ND ;
Cook, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (43) :15289-15294
[4]   MICROSATELLITE ANALYSER (MSA): a platform independent analysis tool for large microsatellite data sets [J].
Dieringer, D ;
Schlotterer, C .
MOLECULAR ECOLOGY NOTES, 2003, 3 (01) :167-169
[5]   Genetic differentiation of Vigna species by RAPD, URP and SSR markers [J].
Dikshit, H. K. ;
Jhang, T. ;
Singh, N. K. ;
Koundal, K. R. ;
Bansal, K. C. ;
Chandra, N. ;
Tickoo, J. L. ;
Sharma, T. R. .
BIOLOGIA PLANTARUM, 2007, 51 (03) :451-457
[6]  
ELLWOOD SR, 2006, THEOR APPL, P1
[7]   Isolation by distance, web service [J].
Jensen, JL ;
Bohonak, AJ ;
Kelley, ST .
BMC GENETICS, 2005, 6 (1)
[8]   Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay [J].
Joshi, P. ;
Dhawan, V. .
BIOLOGIA PLANTARUM, 2007, 51 (01) :22-26
[9]   MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].
Kumar, S ;
Tamura, K ;
Nei, M .
BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163
[10]   Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins [J].
Mirali, N. ;
El-Khouri, S. ;
Rizq, F. .
BIOLOGIA PLANTARUM, 2007, 51 (04) :660-666
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