Construction of a reporter vector for analysis of bidirectional transcriptional activity of retrovirus LTR

被引：25
作者：
Arpin-Andre, Charlotte [1 ,2 ]
Laverdure, Sylvain [1 ,2 ,3 ]
Barbeau, Benoit [4 ,5 ]
Gross, Antoine [1 ,2 ]
Mesnard, Jean-Michel [1 ,2 ]
机构：
[1] Univ Montpellier I, Ctr Etud Agents Pathogenes & Biotechnol Sante CPB, F-34006 Montpellier, France
[2] CNRS, CPBS, UM5236, F-34293 Montpellier, France
[3] E Carolina Univ, Dept Microbiol & Immunol, Greenville, NC 27834 USA
[4] Univ Quebec, Dept Sci Biol, Montreal, PQ H2X 3X8, Canada
[5] Univ Quebec, Ctr Rech BioMed, Montreal, PQ H2X 3X8, Canada
来源：
PLASMID | 2014年 / 74卷
关键词：
HIV-1; HTLV-1; Bidirectional promoter; Antisense transcription; Tat; Tax; HIV-1 ANTISENSE TRANSCRIPTION; ZIPPER FACTOR HBZ; CELLULAR TRANSCRIPTION; PROTEIN; HTLV-1; RNA; STRAND; TAX; EXPRESSION; PROMOTER;
D O I：
10.1016/j.plasmid.2014.06.001
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirectional promoter, which activity was controlled by NF-kappa B- and Sp1-binding sites in both orientations. We next analyzed with this reporter vector the bidirectional promoter activity of the HTLV-1 LTR and showed that this LTR also possessed a bidirectional transcriptional activity. Interestingly, Sp1-binding elements were also involved in the control of HTLV-1 bidirectional transcription. Moreover, both retroviral trans-activators, Tat and Tax, could preferentially activate sense transcription with no or limited effect on the extent of antisense transcription. We also cloned into this plasmid the MLV LTR and found that the LTR of a simple retrovirus also possessed bidirectional transcriptional activity. This reporter vector represents a powerful tool to analyze the bidirectional transcriptional activity of retrovirus LTRs. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：45 / 51
页数：7
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