Toxicity of fullerene and nanosilver nanomaterials against bacteria associated to the body surface of the estuarine worm Laeonereis acuta (Polychaeta, Nereididae)

This study analyzed the growth and biochemical responses of six bacterial colonies isolated from the mucus of the estuarine polychaeta Laeonereis acuta (Nereididae) after exposure to a water suspension of fullerene (nC(60)) and nanosilver (nAg) separately (0.01; 0.10; and 1.00 mg/L) and together (0.01; 0.10; and 1.00 mg/L of nanosilver and 1.00 mg/L of fullerene added to each nAg concentration). Exposures were performed in darkness during 24 h and then samples were taken from the worms and inoculated on agar during 24 h to analyze colonies growth. After this the material was analyzed biochemically. Colonies growth (tested by wet biomass weight) was inhibited at 0.01 and 0.10 mg/L of nAg and 0.01 and 0.10 mg/L nAg + constant 1.00 mg/L of nC(60) (p < 0.05). Lipid peroxidation damage was significant from the control for the concentrations of 0.01 and 0.10 mg/L of nC(60) and glutathione-S-transferase (GST) activity was significantly higher for the concentration of 1.00 mg/L mg/L nAg + constant 1.00 mg/L of nC(60) (P < 0.05). Although nC(60) did not induced growth inhibition, it triggered lipid peroxidation alone and increased GST activity together with nAg.(60) Contrary to nC(60), nanosilver inhibited bacterial growth, although the biochemical measurements indicate that this response is not due to reactive oxygen species generation. (C) 2014 Elsevier Ltd. All rights reserved.