Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees

被引：17

作者：

Vakil, Moinuddin M. A. ^{[1
]}

Mendhulkar, Vijay D. ^{[1
]}

机构：

[1] Inst Sci, Bombay 400032, Maharashtra, India

来源：

BOTANICAL STUDIES | 2013年 / 54卷

关键词：

Active constituents; Fungal elicitors; HPLC analysis; Secondary metabolites; Tissue culture techniques; HIGH-AFFINITY BINDING; PLASMA-MEMBRANE; FUNGAL ELICITATION; SOYBEAN MEMBRANES; OXIDATIVE BURST; TISSUE CULTURES; PROTEIN-KINASES; PLANT-CELLS; DEFENSE; ACTIVATION;

D O I：

10.1186/1999-3110-54-49

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Andrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. Cell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with Aspergillus niger and Penicillium expansum elicitors to enhance the synthesis of andrographolide, the bioactive constituent of A. paniculata. Result: The elicitation treatment with fungal elicitors (A. niger and P. expansum) was observed to be most suitable for eliciting andrographolide production in the culture. The quantification of andrographolide was done using High Performance Liquid Chromatography (HPLC) technique. A. niger extract (1.5 ml with 10 days treatment duration) revealed 6.94 fold increase in andrographolide content (132 mu g) which was higher than the control (19 mu g). P. expansum elicitor (0.6% with 8 days treatment duration) could reveal 6.23 fold enhancement in andrographolide content (81.0 mu g) over control (13 mu g). Conclusion: The results obtained reveal that the longer treatment duration is most favorable for the elicitation of andrographolide using both the fungal elicitors.

引用

页数：8