RADIATION DOSE ESTIMATION BY AUTOMATED CYTOGENETIC BIODOSIMETRY

被引：16

作者：

Rogan, Peter K. ^{[1
,2
]}

Li, Yanxin ^{[1
]}

Wilkins, Ruth C. ^{[3
]}

Flegal, Farrah N. ^{[4
]}

Knoll, Joan H. M. ^{[2
,5
]}

机构：

[1] Univ Western Ontario, Schulich Sch Med & Dent, Dept Biochem, London, ON N6A 5C1, Canada

[2] Cytognomix Inc, 60 North Ctr Rd,Box 27052, London, ON N5X 3X5, Canada

[3] Hlth Canada, Consumer & Clin Radiat Protect Bur, 775 Brookfield Rd, Ottawa, ON K1A 1C1, Canada

[4] Canadian Nucl Labs Radiobiol & Hlth, Plant Rd,Chalk River, Chalk River, ON K0J 1J0, Canada

[5] Univ Western Ontario, Schulich Sch Med & Dent, Dept Pathol & Lab Med, London, ON N6A 5C1, Canada

来源：

RADIATION PROTECTION DOSIMETRY | 2016年 / 172卷 / 1-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHROMOSOMES; TRIAGE;

D O I：

10.1093/rpd/ncw161

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The dose from ionizing radiation exposure can be interpolated from a calibration curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As DC counts are manually determined, there is an acute need for accurate, fully automated biodosimetry calibration curve generation and analysis of exposed samples. Software, the Automated Dicentric Chromosome Identifier (ADCI), is presented which detects and discriminates DCs from monocentric chromosomes, computes biodosimetry calibration curves and estimates radiation dose. Images of metaphase cells from samples, exposed at 1.4-3.4Gy, that had been manually scored by two reference laboratories were reanalyzed with ADCI. This resulted in estimated exposures within 0.4-1.1Gy of the physical dose. Therefore, ADCI can determine radiation dose with accuracies comparable to standard triage biodosimetry. Calibration curves were generated from metaphase images in similar to 10 h, and dose estimations required similar to 0.8 h per 500 image sample. Running multiple instances of ADCI may be an effective response to a mass casualty radiation event.

引用

页码：207 / 217

页数：11

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