Functional Characteristics Analysis of Dehydrins in Larix kaempferi under Osmotic Stress

被引:13
作者
Wang, Xuechun [1 ]
Zhang, Meng [1 ]
Xie, Baohui [1 ]
Jiang, Xiangning [1 ,2 ]
Gai, Ying [1 ,2 ]
机构
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
[2] Chinese Forestry Adm, Natl Engn Lab Tree Breeding, Tree & Ornamental Plant Breeding & Biotechnol Lab, Beijing 100083, Peoples R China
关键词
dehydrin; osmotic stress; DNA binding; Larix kaempferi;
D O I
10.3390/ijms22041715
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dehydrins (DHN) belong to the late embryogenesis abundant II family and have been found to enhance plant tolerance to abiotic stress. In the present study, we reported four DHNs in Larix kaempferi (LkDHN) which were identified from the published transcriptome. Alignment analysis showed that these four LkDHNs shared close relationships and belonged to SK3-type DHNs. The electrophoretic mobility shift assay indicated that these four LkDHNs all possess sequence-independent binding capacity for double-strands DNAs. The subcellular localizations of the four LkDHNs were in both the nucleus and cytoplasm, indicating that these LkDHNs enter the nucleus to exert the ability to bind DNA. The preparation of tobacco protoplasts with different concentrations of mannitol showed that LkDHNs enhanced the tolerance of plant cells under osmotic stress. The overexpression of LkDHNs in yeasts enhanced their tolerance to osmotic stress and helped the yeasts to survive severe stress. In addition, LkDHNs in the nucleus of salt treated tobacco increased. All of these results indicated that the four LkDHNs help plants survive from heavy stress by participating in DNA protection. These four LKDHNs played similar roles in the response to osmotic stress and assisted in the adaptation of L. kaempferi to the arid and cold winter of northern China.
引用
收藏
页码:1 / 11
页数:11
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