Trichostatin A modulates expression of p21waf1/cip1, Bcl-xL, ID1, ID2, ID3, CRABP, GATA-2, hsp86 and TFIID/TAFII31 mRNA in human lung adenocarcinoma cells

被引：48
作者：
Eickhoff, B
Rüller, S
Laue, T
Köhler, G
Stahl, C
Schlaak, M
van der Bosch, J
机构：
[1] Res Ctr Borstel, Div Expt Immunopharmacol, D-23845 Borstel, Germany
[2] Artus GmbH, D-20459 Hamburg, Germany
[3] Univ Hamburg, Fac Biol, Hamburg, Germany
来源：
BIOLOGICAL CHEMISTRY | 2000年 / 381卷 / 02期
关键词：
apoptosis; cDNA array; complex hybridization; histone deacetylase inhibitor; Taqman;
D O I：
10.1515/BC.2000.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lung adenocarcinoma cells treated for 16 h with trichostatin A (TSA), an inhibitor of histone deacetylases, and untreated cells were analyzed with respect to differential gene expression. Complex hybridization of cDNA arrays:revealed repression of Bcl-xL, CRABP and TFIID/TBFII31 as well as induction of p21(waf1/cip1) GATA-2, hsp86, ID1, ID2 and ID3 mRNA expression, which could be verified by Northern blotting. ID2 induction was further confirmed by Taqman realtime quantitative RT-PCR. The described alterations of gene expression due to TSA renders the lung adenocarcinoma cells susceptible to induction of apoptosis.
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页码：107 / 112
页数：6
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