A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification

被引：9

作者：

Ding, Baomiao ^{[1
]}

Liu, Chenguang ^{[2
]}

Wu, Qinghua ^{[1
]}

Wang, Yun ^{[2
]}

Li, Li ^{[2
]}

Yang, Hualin ^{[2
]}

机构：

[1] Yangtze Univ, Coll Life Sci, Jingchu Food Res & Dev Ctr, Jingzhou 434025, Hubei, Peoples R China

[2] Yangtze Univ, Coll Life Sci, Edible & Med Fungi Res Ctr, Jingzhou 434025, Hubei, Peoples R China

来源：

ANALYTICAL SCIENCES | 2018年 / 34卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Mercury ion detection; G-quadruplex DNA; exonuclease III; T-Hg-T mismatches; HYBRIDIZATION CHAIN-REACTION; AQUEOUS-SOLUTION; QUADRUPLEX DNA; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; HG2+; SENSOR; BINDING; PROBES; DYES;

D O I：

10.2116/analsci.34.259

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury ion detection.

引用

页码：259 / 261

页数：3

共 50 条

[41] A label-free Exonuclease I-assisted fluorescence aptasensor for highly selective and sensitive detection of silver ions
Wei, Zhiwen
Lan, Yifeng
Zhang, Chao
Jia, Juan
Niu, Weifen
Wei, Yanli
Fu, Shanlin
Yun, Keming
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2021, 260
[42] Quadratic recycling amplification for label-free and sensitive visual detection of HIV DNA
Zhou, Wenjiao
Gong, Xue
Xiang, Yun
Yuan, Ruo
Chai, Yaqin
BIOSENSORS & BIOELECTRONICS, 2014, 55 : 220 - 224
[43] Label-free and ultrasensitive electrochemical detection of nucleic acids based on an exonuclease III-assisted target recycling amplification strategy using a heated gold disk electrode
Cheng, Yanggang
Liu, Minglu
Wang, Fangfang
ANALYTICAL METHODS, 2024, 16 (45) : 7736 - 7743
[44] Label-Free and Sensitive Electrochemical Detection of DNA Methylation Damage Based on DNA Glycosylase Recognition and Excision Coupled with Exonuclease III Amplification
Zhang, Qing-Zheng
Su, Chun-Bo
Shi, San-Jun
Gao, Yue
Huang, Rong-Fu
JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2023, 170 (11)
[45] A novel fluorescent aptasensor based on exonuclease-assisted triple recycling amplification for sensitive and label-free detection of aflatoxin B1
Wu, Hao
Wang, Hongyong
Wu, Jun
Han, Guoqing
Liu, Yaling
Zou, Pei
Journal of Hazardous Materials, 2021, 415
[46] A novel fluorescent aptasensor based on exonuclease-assisted triple recycling amplification for sensitive and label-free detection of aflatoxin B1
Wu, Hao
Wang, Hongyong
Wu, Jun
Han, Guoqing
Liu, Yaling
Zou, Pei
JOURNAL OF HAZARDOUS MATERIALS, 2021, 415
[47] A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction
Tian, Yang
Wang, Yue
Xu, Yan
Liu, Yang
Li, Di
Fan, Chunhai
SCIENCE CHINA-CHEMISTRY, 2015, 58 (03) : 514 - 518
[48] Exonuclease III-assisted cascade signal amplification strategy for label-free and ultrasensitive electrochemical detection of nucleic acids
Xiong, Erhu
Yan, Xiaoxia
Zhang, Xiaohua
Liu, Yunqing
Zhou, Jiawan
Chen, Jinhua
BIOSENSORS & BIOELECTRONICS, 2017, 87 : 732 - 736
[49] A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction
Yang Tian
Yue Wang
Yan Xu
Yang Liu
Di Li
Chunhai Fan
Science China Chemistry, 2015, 58 : 514 - 518
[50] A novel exonuclease III-aided amplification assay for lysozyme based on graphene oxide platform
Chen, Chunfei
Zhao, Jingjin
Jiang, Jianhui
Yu, Ruqin
TALANTA, 2012, 101 : 357 - 361

← 1 2 3 4 5 →