A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification

被引：9

作者：

Ding, Baomiao ^{[1
]}

Liu, Chenguang ^{[2
]}

Wu, Qinghua ^{[1
]}

Wang, Yun ^{[2
]}

Li, Li ^{[2
]}

Yang, Hualin ^{[2
]}

机构：

[1] Yangtze Univ, Coll Life Sci, Jingchu Food Res & Dev Ctr, Jingzhou 434025, Hubei, Peoples R China

[2] Yangtze Univ, Coll Life Sci, Edible & Med Fungi Res Ctr, Jingzhou 434025, Hubei, Peoples R China

来源：

ANALYTICAL SCIENCES | 2018年 / 34卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Mercury ion detection; G-quadruplex DNA; exonuclease III; T-Hg-T mismatches; HYBRIDIZATION CHAIN-REACTION; AQUEOUS-SOLUTION; QUADRUPLEX DNA; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; HG2+; SENSOR; BINDING; PROBES; DYES;

D O I：

10.2116/analsci.34.259

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury ion detection.

引用

页码：259 / 261

页数：3

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