A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification

被引：9
|
作者：
Ding, Baomiao [1 ]
Liu, Chenguang [2 ]
Wu, Qinghua [1 ]
Wang, Yun [2 ]
Li, Li [2 ]
Yang, Hualin [2 ]
机构：
[1] Yangtze Univ, Coll Life Sci, Jingchu Food Res & Dev Ctr, Jingzhou 434025, Hubei, Peoples R China
[2] Yangtze Univ, Coll Life Sci, Edible & Med Fungi Res Ctr, Jingzhou 434025, Hubei, Peoples R China
来源：
ANALYTICAL SCIENCES | 2018年 / 34卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Mercury ion detection; G-quadruplex DNA; exonuclease III; T-Hg-T mismatches; HYBRIDIZATION CHAIN-REACTION; AQUEOUS-SOLUTION; QUADRUPLEX DNA; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; HG2+; SENSOR; BINDING; PROBES; DYES;
D O I：
10.2116/analsci.34.259
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury ion detection.
引用
收藏
页码：259 / 261
页数：3
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