Establishing a Cell Model for Studying the Interaction of the Pou5f1 (Oct4) Promoter with the Genetic Environment

被引：1

作者：

Kuzmin, A. A. ^{[1
]}

Ermakova, V. V. ^{[1
]}

Potapenko, E. V. ^{[1
]}

Ostroverkhova, M. G. ^{[1
]}

Guriev, N. A. ^{[1
]}

Tomilin, A. N. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia

来源：

RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY | 2020年 / 51卷 / 06期

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

Pou5f1; cis-regulatory elements; embryonic stem cells; pluripotency; CRISPR; Cas9; Rosa26; ePromoter; REGULATORY ELEMENT; EXPRESSION; NAIVE;

D O I：

10.1134/S106236042006003X

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pou5f1 (Oct4), a key gatekeeper of cellular pluripotency, has little been studied with regard to roles of its regulatory cis-elements in transcriptional control of neighboring genes. In this study, bi-allelic deletion of the Pou5f1 promoter with simultaneous rescue of mouse embryonic stem cell (ESC) self-renewal by a 9.8-kb fragment of Pou5f1 knocked into the Rosa26 locus has been performed. The results confirm the previously established Pou5f1 gene boundaries, whereas the generated ESCs will be indispensable for further studies of the Pou5f1 promoter in transcriptional regulation of neighbor genes.

引用

页码：410 / 415

页数：6

共 13 条

[1] Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells [J].

Bressan, Raul Bardini ;

Dewari, Pooran Singh ;

Kalantzaki, Maria ;

Gangoso, Ester ;

Matjusaitis, Mantas ;

Garcia-Diaz, Claudia ;

Blin, Carla ;

Grant, Vivien ;

Bulstrode, Harry ;

Gogolok, Sabine ;

Skarnes, William C. ;

Pollard, Steven M. .

DEVELOPMENT, 2017, 144 (04) :635-648

[2] The genetic polymorphisms of POU5F1 gene are associated with psoriasis vulgaris in Chinese [J].

Chang, Yun-Ting ;

Hsu, Chih-Yi ;

Chou, Chan-Te ;

Lin, Ming-Wei ;

Shiao, Yu-Ming ;

Tsai, Chang-Youh ;

Yu, Chia-Wen ;

Lee, Yu-Fen ;

Huang, Cheng-Hung ;

Chen, Chih-Chiang ;

Lee, Ding-Dar ;

Wang, Wen-Jen ;

Liu, Han-Nan ;

Tsai, Shih-Feng .

JOURNAL OF DERMATOLOGICAL SCIENCE, 2007, 46 (02) :153-156

[3] Distinct Enhancer Activity of Oct4 in Naive and Primed Mouse Pluripotency [J].

Choi, Hyun Woo ;

Joo, Jin Young ;

Hong, Yean Ju ;

Kim, Jong Soo ;

Song, Hyuk ;

Lee, Jeong Woong ;

Wu, Guangming ;

Schoeler, Hans R. ;

Do, Jeong Tae .

STEM CELL REPORTS, 2016, 7 (05) :911-926

[4] Genome-wide characterization of mammalian promoters with distal enhancer functions [J].

Dao, Lan T. M. ;

Galindo-Albarran, Ariel O. ;

Castro-Mondragon, Jaime A. ;

Andrieu-Soler, Charlotte ;

Medina-Rivera, Alejandra ;

Souaid, Charbel ;

Charbonnier, Guillaume ;

Griffon, Aurelien ;

Vanhille, Laurent ;

Stephen, Tharshana ;

Alomairi, Jaafar ;

Martin, David ;

Torres, Magali ;

Fernandez, Nicolas ;

Soler, Eric ;

van Helden, Jacques ;

Puthier, Denis ;

Spicuglia, Salvatore .

NATURE GENETICS, 2017, 49 (07) :1073-+

[5]

Diao YR, 2017, NAT METHODS, V14, P629, DOI [10.1038/NMETH.4264, 10.1038/nmeth.4264]

[6] Pioneer transcription factors in cell reprogramming [J].

Iwafuchi-Doi, Makiko ;

Zaret, Kenneth S. .

GENES & DEVELOPMENT, 2014, 28 (24) :2679-2692

[7] Reduced Oct4 Expression Directs a Robust Pluripotent State with Distinct Signaling Activity and Increased Enhancer Occupancy by Oct4 and Nanog [J].

Karwacki-Neisius, Violetta ;

Goeke, Jonathan ;

Osorno, Rodrigo ;

Halbritter, Florian ;

Ng, Jia Hui ;

Weisse, Andrea Y. ;

Wong, Frederick C. K. ;

Gagliardi, Alessia ;

Mullin, Nicholas P. ;

Festuccia, Nicola ;

Colby, Douglas ;

Tomlinson, Simon R. ;

Ng, Huck-Hui ;

Chambers, Ian .

CELL STEM CELL, 2013, 12 (05) :531-545

[8] Oct4 is required for primordial germ cell survival [J].

Kehler, J ;

Tolkunova, E ;

Koschorz, B ;

Pesce, M ;

Gentile, L ;

Boiani, M ;

Lomelí, H ;

Nagy, A ;

McLaughlin, KJ ;

Schöler, HR ;

Tomilin, A .

EMBO REPORTS, 2004, 5 (11) :1078-1083

[9] In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states [J].

Neagu, Alex ;

van Genderen, Emiel ;

Escudero, Irene ;

Verwegen, Lucas ;

Kurek, Dorota ;

Lehmann, Johannes ;

Stel, Jente ;

Dirks, Rene A. M. ;

van Mierlo, Guido ;

Maas, Alex ;

Eleveld, Cindy ;

Ge, Yang ;

den Dekker, Alexander. T. ;

Brouwer, Rutger W. W. ;

van IJcken, Wilfred F. J. ;

Modic, Miha ;

Drukker, Micha ;

Jansen, Joop H. ;

Rivron, Nicolas C. ;

Baart, Esther B. ;

Marks, Hendrik ;

ten Berge, Derk .

NATURE CELL BIOLOGY, 2020, 22 (05) :534-545

[10] Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4 [J].

Nichols, J ;

Zevnik, B ;

Anastassiadis, K ;

Niwa, H ;

Klewe-Nebenius, D ;

Chambers, I ;

Schöler, H ;

Smith, A .

CELL, 1998, 95 (03) :379-391

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