Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs

被引：0

作者：

Wang, Yu Winston ^{[1
]}

Khan, Altaz ^{[1
]}

Som, Madhura ^{[2
]}

Leigh, Steven Y. ^{[1
]}

Wang, Danni ^{[1
]}

Chen, Ye ^{[1
]}

McVeigh, Patrick ^{[3
,4
]}

Wilson, Brian C. ^{[3
,4
]}

Liu, Jonathan T. C. ^{[1
]}

机构：

[1] SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA

[2] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA

[3] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada

[4] Princess Margaret Canc Ctr, Toronto, ON, Canada

来源：

TRANSLATIONAL BIOPHOTONICS | 2014年 / 9155卷

关键词：

Raman spectroscopy; SERS nanotags; multiplexed molecular imaging; cancer biomarkers; early detection; point-of-care diagnostics; real-time detection;

D O I：

10.1117/12.2057716

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted to cell-surface biomarkers in fresh tissues. Ex vivo and in vivo experiments were performed to optimize our strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5 min) topical application of SERS NPs on tissues. The simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations, potentially enabling point-of-care surgical guidance or early disease detection.

引用

页数：3

共 6 条

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