Guide to the Assessment of Mature Liver Gene Expression in Stem Cell-Derived Hepatocytes

被引：47

作者：

Zabulica, Mihaela ^{[1
]}

Srinivasan, Raghuraman C. ^{[1
]}

Vosough, Massoud ^{[2
]}

Hammarstedt, Christina ^{[1
]}

Wu, Tingting ^{[1
]}

Gramignoli, Roberto ^{[1
]}

Ellis, Ewa ^{[3
]}

Kannisto, Kristina ^{[4
]}

de l'Hortet, Alexandra Collin ^{[5
]}

Takeishi, Kazuki ^{[5
]}

Soto-Gutierrez, Alejandro ^{[5
]}

Strom, Stephen C. ^{[1
]}

机构：

[1] Karolinska Inst, Dept Lab Med, Div Pathol, S-14186 Stockholm, Sweden

[2] Royan Inst Stem Cell Biol, Tehran, Iran

[3] Karolinska Inst, Clin Sci Intervent & Technol CLINTEC, Stockholm, Sweden

[4] Karolinska Inst, Clin Res Ctr, Dept Lab Med, Stockholm, Sweden

[5] Univ Pittsburgh, Dept Pathol, Pittsburgh, PA USA

来源：

STEM CELLS AND DEVELOPMENT | 2019年 / 28卷 / 14期

基金：

美国国家卫生研究院;

关键词：

induced pluripotent stem cells; hepatic differentiation; stem cell-derived hepatocytes; mature liver gene expression; fetal liver gene expression; DRUG-METABOLIZING-ENZYMES; PREGNANE X-RECEPTOR; 3D CULTURE-SYSTEMS; 3-DIMENSIONAL CULTURE; HNF4-ALPHA ISOFORMS; RESPONSIVE ENHANCER; ONTOGENY; DIFFERENTIATION; TRANSPLANTATION; MATURATION;

D O I：

10.1089/scd.2019.0064

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Differentiation of stem cells to hepatocyte-like cells (HLCs) holds great promise for basic research, drug and toxicological investigations, and clinical applications. There are currently no protocols for the production of HLCs from stem cells, such as embryonic stem cells or induced pluripotent stem cells, that produce fully mature hepatocytes with a wide range of mature hepatic functions. This report describes a standard method to assess the maturation of stem cell-derived HLCs with a moderately high-throughput format, by analysing liver gene expression by quantitative RT-qPCR. This method also provides a robust data set of the expression of 62 genes expressed in normal liver, generated from 17 fetal and 25 mature human livers, so that investigators can quickly and easily compare the expression of these genes in their stem cell-derived HLCs with the values obtained in authentic fetal and mature human liver. The simple methods described in this study will provide a quick and accurate assessment of the efficacy of a differentiation protocol and will help guide the optimization of differentiation conditions.

引用

页码：907 / 919

页数：13

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