Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow

被引：16

作者：

Yang, Hao ^{[1
,2
,3
]}

Li, Ning ^{[1
,2
,3
]}

Du, Yu ^{[1
,2
,3
]}

Tong, Chunfang ^{[1
,2
,3
]}

Lu, Shouqin ^{[1
,2
,3
]}

Hu, Jinrong ^{[1
,2
,3
]}

Zhang, Yan ^{[1
,2
,3
]}

Long, Mian ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Natl Micrograv Lab, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China

[3] Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2017年 / 351卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Neutrophils; Liver sinusoids; Shear flow; Adhesive molecules; Kupffer cells; INFLAMED LIVER; HEPATIC MICROCIRCULATION; LEUKOCYTE RECRUITMENT; EXTRACELLULAR-MATRIX; INJURY; SITES; INFECTION; SEQUESTRATION; INFLAMMATION; LYMPHOCYTES;

D O I：

10.1016/j.yexcr.2017.01.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Neutrophil (polymorphonuclear leukocyte, PMN) recruitment in the liver sinusoid takes place in almost all liver diseases and contributes to pathogen clearance or tissue damage. While PMN rolling unlikely appears in liver sinusoids and Mac-1 or CD44 is assumed to play respective roles during in vivo local or systematic inflammatory stimulation, the regulating mechanisms of PMN adhesion and crawling dynamics are still unclear from those in vivo studies. Here we developed a two-dimensional in vitro sinusoidal model with primary liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) to investigate TNF-alpha-induced PMN recruitment under shear flow. Our data demonstrated that LFA-1 dominates the static or shear resistant adhesion of PMNs while Mac-1 decelerates PMN crawling on LSEC monolayer. Any one of LFA-1, Mac-1, and CD44 molecules is not able to work effectively for mediating PMN transmigration across LSEC monolayer. The presence of KCs only affects the randomness of PMN crawling. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids.

引用

页码：91 / 99

页数：9

共 44 条

[1] Chronic alcohol intoxication induces hepatic injury through enhanced macrophage inflammatory protein-2 production and intercellular adhesion molecule-1 expression in the liver [J].