Membrane capacitance techniques to monitor granule exocytosis in neutrophils

被引：44
作者：
Lollike, K
Lindau, M
机构：
[1] Rigshosp, Granulocyte Res Lab, Dept Hematol, Finsen Ctr, DK-2100 Copenhagen, Denmark
[2] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 232卷 / 1-2期
关键词：
cell-attached; whole-cell; secretion; fusion pore; patch-clamp; membrane fusion;
D O I：
10.1016/S0022-1759(99)00169-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cell membranes behave like electrical capacitors and changes in cell capacitance therefore reflect changes in the cell area. Monitoring capacitance can thus be used to study dynamic cellular phenomenon involving rapid changes in cell surface, such as exo- and/or endocytosis, In this review focus is on the use of capacitance techniques to study exocytosis in human neutrophils, We compare the whole-cell and the cell-attached capacitance techniques, and we review the complete literature dealing with capacitance measurements in human neutrophils. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：111 / 120
页数：10
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