A method for identification of HIV gp140 binding memory B cells in human blood

被引：171

作者：

Scheid, Johannes F. ^{[2
]}

Mouquet, Hugo ^{[2
]}

Feldhahn, Niklas ^{[2
]}

Walker, Bruce D. ^{[4
]}

Pereyra, Florencia ^{[4
]}

Cutrell, Emily ^{[4
]}

Seaman, Michael S. ^{[5
]}

Mascola, John R. ^{[3
]}

Wyatt, Richard T. ^{[3
]}

Wardemann, Hedda ^{[6
]}

Nussenzweig, Michel C. ^{[1
,2
]}

机构：

[1] Rockefeller Univ, HHMI, New York, NY 10065 USA

[2] Rockefeller Univ, Lab Mol Immunol, New York, NY 10065 USA

[3] NIH, Vaccine Res Ctr, Bethesda, MD 20892 USA

[4] Mass Gen Hosp, Partners AIDS Rsearch Ctr, Charlestown, MA 02129 USA

[5] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA

[6] Max Planck Inst Infect Biol, D-10117 Berlin, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 343卷 / 02期

关键词：

HIV; Memory B cells; gp140; MONOCLONAL-ANTIBODIES; FREQUENCIES;

D O I：

10.1016/j.jim.2008.11.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immnodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Nail. Acad. Sci. U. S. A. 88, 10134-10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C.. Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191-195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：65 / 67

页数：3

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