Expressing CS6 of enterotoxigenic Escherichia coli by attenuated Shigella flexneri 2a

被引：0

作者：

Luo, G

Li, SQ

Wang, LC

Zhao, Y

Zheng, JP

Duan, HQ

Zhang, ZS ^{[1
]}

机构：

[1] Beijing Inst Biotechnol, Beijing 100071, Peoples R China

[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2004年 / 31卷 / 02期

关键词：

enterotoxigenic Escherichia coli; CS6; Shigella flexner; host-plasmid letlhal balancing system; live vector vaccine;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A host-plasmid lethal balancing system was constructed based on asd gene in an avirulent strain of S.flexneri to express coli surface antigen 6 of enterotoxigenic Escherichia coli. The results of Western-blotting demonstrated that avirulant strain of S. flexneri FWL01 expressed CS6 steadily. Immunofluorescence analysis showed that S.flexnerl FWL01 carrying the plasmid pZLG6 can be excited fluorescence on its surface. Antibodies against CS6 and LPS of Shigella can be detected in sera of mice immunized with recombinant bacteria either orogastrically (o.g.) or intranasally (i.n.); simultaneously slgA against CS6 can also be detected in the intestine. This is helpful for constructing multivalent recombinant vaccine for prevention of bacterial diarrhea.

引用

页码：154 / 158

页数：5

共 20 条

[1] INTESTINAL ANTIBODY-RESPONSE AFTER ORAL IMMUNIZATION WITH A PROTOTYPE CHOLERA-B SUBUNIT - COLONIZATION FACTOR ANTIGEN ENTEROTOXIGENIC ESCHERICHIA-COLI VACCINE [J].