Size-exclusion chromatography study of the molecular-weight distribution of γ-irradiated pullulan

被引：6

作者：

Shingel, KI ^{[1
]}

Tsarenkov, VM ^{[1
]}

Petrov, PT ^{[1
]}

机构：

[1] Belmedpreparaty Pharmaceut Co, Sci Pharmaceut Ctr, Minsk 220001, BELARUS

来源：

CARBOHYDRATE RESEARCH | 2000年 / 324卷 / 04期

关键词：

gamma-irradiated pullulan; high-performance size-exclusion chromatography; molecular-weight distribution;

D O I：

10.1016/S0008-6215(99)00305-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polyelectrolyte behavior of gamma-irradiated pullulan in aqueous solutions leads to secondary effects of adsorption on a Shodex OHPak KB 806 column gel during size-exclusion chromatography. Suppression of the polyelectrolyte properties of gamma-irradiated pullulan is achieved by using a 0.05 M aqueous solution of NaH2PO4 (pH 4.95) as the mobile phase. Under these conditions, adequate molecular-weight distributions of gamma-irradiated pullulan samples are obtained. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：283 / 287

页数：5

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