Cell-free DNA detected by "liquid biopsy" as a potential prognostic biomarker in early breast cancer

被引:29
作者
Maltoni, Roberta [1 ]
Casadio, Valentina [2 ]
Ravaioli, Sara [2 ]
Foca, Flavia [3 ]
Tumedei, Maria Maddalena [2 ]
Salvi, Samanta [2 ]
Martignano, Filippo [2 ]
Calistri, Daniele [2 ]
Rocca, Andrea [1 ]
Schirone, Alessio [1 ]
Amadori, Dino [1 ]
Bravaccini, Sara [2 ]
机构
[1] IRCCS, Ist Sci Romagnolo Studio Cura Tumori IRST, Dept Med Oncol, Meldola, Italy
[2] IRCCS, Ist Sci Romagnolo Studio Cura Tumori IRST, Biosci Lab, Meldola, Italy
[3] IRCCS, Ist Sci Romagnolo Studio Cura Tumori IRST, Unit Biostat & Clin Trials, Meldola, Italy
关键词
CF-DNA; HER2; PI3KCA; prognosis; breast cancer subtypes; CIRCULATING TUMOR DNA; HER2; AMPLIFICATION; PLASMA; SERUM;
D O I
10.18632/oncotarget.15120
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
As conventional biomarkers for defining breast cancer (BC) subtypes are not always capable of predicting prognosis, search for new biomarkers which can be easily detected by liquid biopsy is ongoing. It has long been known that cell-free DNA (CF-DNA) could be a promising diagnostic and prognostic marker in different tumor types, although its prognostic value in BC is yet to be confirmed. This retrospective study evaluated the prognostic role of CF-DNA quantity and integrity of HER2, MYC, BCAS1 and PI3KCA, which are frequently altered in BC. We collected 79 serum samples before surgery from women at first diagnosis of BC at Forli Hospital (Italy) from 2002 to 2010. Twenty-one relapsed and 58 non-relapsed patients were matched by subtype and age. Blood samples were also collected from 10 healthy donors. All samples were analyzed by Real Time PCR for CF-DNA quantity and integrity of all oncogenes. Except for MYC, BC patients showed significantly higher median values of CF-DNA quantity (ng) than healthy controls, who had higher integrity and lower apoptotic index. A difference nearing statistical significance was observed for HER2 short CF-DNA (p = 0.078, AUC value: 0.6305). HER2 short CF-DNA showed an odds ratio of 1.39 for disease recurrence with p = 0.056 (95% CI 0.991-1.973). Our study suggests that CF-DNA detected as liquid biopsy could have great potential in clinical practice once demonstration of its clinical validity and utility has been provided by prospective studies with robust assays.
引用
收藏
页码:16642 / 16649
页数:8
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