Determination of antioxidant activity and phenolic compounds of Muntingia calabura Linn. peel by HPLC-DAD and UPLC-ESI-MS/MS

被引：21

作者：

Rotta, Eliza Mariane ^{[1
]}

Isidoro Haminiuk, Charles Windson ^{[2
]}

Maldaner, Liane ^{[1
]}

Visentainer, Jesui Vergilio ^{[1
]}

机构：

[1] State Univ Maringa UEM, Dept Chem, Ave Colombo 5790, BR-87020900 Maringa, PR, Brazil

[2] Fed Technol Univ Parana UTFPR, Campus Campo Mourao, BR-87301899 Campo Mourao, PR, Brazil

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2017年 / 52卷 / 04期

关键词：

Antioxidant source; extraction conditions; fruit peel; HPLC-DAD; UPLC-ESI-MS; MS; TROPICAL FRUITS; BIOACTIVE COMPOUNDS; EXTRACTION; CAPACITY; LEAVES; SOLVENT; YUCATAN; RESIDUE; PARTS;

D O I：

10.1111/ijfs.13359

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Antioxidant activity in Muntingia calabura Linn. peel was evaluated by DPPH radical, ORAC, ABTS cation radical, FRAP assays and total phenolic contents by different extraction conditions. In addition, a method for determination of phenolic compounds in calabura peel samples harvested in Brazil using methanol:water and magnetic stirring as the extraction method, HPLC-DAD and UPLC-ESI-MS/MS analysis were developed. Calabura peel showed antioxidant activity for all extraction conditions and assays evaluated, the most polar solvents being more effective The developed HPLC-DAD method allowed the accurate determination of phenolic compounds, with recoveries in the range of 72-107% and precision values 4%, with exception for chlorogenic acid. Gallic acid was determined at the highest concentration levels, followed by myricetin, ferulic acid and vanillic acid. However, all the five proposed phenolic compounds were identified in calabura peel samples by UPLC-ESI-MS/MS. Thus, calabura peel, an uncommon edible fruit part, can be appointed as a rich source of phenolic compounds.

引用

页码：954 / 963

页数：10

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