Cellular imaging and folate receptor targeting delivery of gum kondagogu capped gold nanoparticles in cancer cells

被引：34

作者：

Kumar, Sathish Sundar Dhilip ^{[1
,2
,5
]}

Mahesh, Ayyavu ^{[3
]}

Antoniraj, M. Gover ^{[1
]}

Rathore, Hanumant Singh ^{[4
]}

Houreld, N. N. ^{[5
]}

Kandasamy, Ruckmani ^{[1
,2
]}

机构：

[1] Anna Univ, Dept Pharmaceut Technol, Ctr Excellence Nanobio Translat Res CENTRE, BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India

[2] Anna Univ, Natl Facil Drug Dev Acad Pharmaceut & Allied Ind, BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India

[3] Madurai Kamaraj Univ, Sch Biol Sci, Ctr Excellence Genom Sci, Madurai 625021, Tamil Nadu, India

[4] Nagaland Cent Univ, Sch Engn & Technol, Dept Biotechnol, Nagaland 797112, India

[5] Univ Johannesburg, Laser Res Ctr, ZA-2028 Johannesburg, South Africa

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 109卷

关键词：

Gold nanoparticles; Polysaccharides; Folic acid; Cellular imaging; Cancer cells; FOLIC-ACID; GREEN SYNTHESIS; CHITOSAN;

D O I：

10.1016/j.ijbiomac.2017.12.069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, the green synthesis of gum kondagogu capped gold nanoparticles (GK-GNPs) was prepared using a naturally available polysaccharide. The anionic gum capped GK-GNPs enabled the successful coupling of folic acid (FA) and fluorescein isothiocyanate (FITC) to produce a fluorescently labelled GNP (F2-GNP). F2-GNPs were further characterized using different physicochemical methods Cellular viability, cellular imaging, and targeted delivery of F2-GNPs were further evaluated in both folate receptor positive (MCF-7) and folate receptor negative (A549) cancer cells. Physicochemical characterization revealed a nanoparticle with a small size (37 nm), smooth surface (surface charge of -23.7 mV), crystallinity of gold nanoparticles and existence of gum kondagogu in the F2-GNPs. Cellular uptake of F2-GNPs indicated a greater affinity towards folate receptor positive cells. This study shows that the F2-GNPs is as an effective nanocarrier for targeted drug delivery and cellular imaging via folate receptors. (C) 2017 Published by Elsevier B.V.

引用

页码：220 / 230

页数：11

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