Synergist bioassays: A simple method for initial metabolic resistance investigation of field Anopheles gambiae s.l. populations

被引:33
作者
Chouaibou, Mouhamadou [1 ]
Zivanovic, Georgina Bingham [2 ]
Knox, Tessa B. [3 ]
Jamet, Helen Pates [2 ]
Bonfoh, Bassirou [1 ]
机构
[1] Ctr Suisse Rech Sci Cote Ivoire CSRS, Abidjan 01, Cote Ivoire
[2] Vestergaard Frandsen, CH-1006 Lausanne, Switzerland
[3] Vestergaard Frandsen, Nairobi, Kenya
基金
英国惠康基金;
关键词
Anopheles gambiae; Insecticide resistance; Metabolic resistance; P450s; Esterases; P-glycoprotein efflux pump; INSECTICIDE RESISTANCE; PIPERONYL BUTOXIDE; P-GLYCOPROTEIN; COTE-DIVOIRE; CULICIDAE; DIPTERA; AFRICA; KDR;
D O I
10.1016/j.actatropica.2013.10.020
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Metabolic resistance and the potential role of permeability-glycoprotein (P-gp) efflux pumps were investigated in a pyrethroid-resistant wild Anopheles gambiae s.l. Tiassale population, using WHO susceptibility assays with deltamethrin (0.05%), with and without pre-exposure to synergists. The synergists used included an inhibitor of P-glycoprotein efflux pumps (verapamil), an inhibitor of esterases (EN 16-5), and an inhibitor of P450s and esterases (piperonyl butoxide). Pre-exposure to verapamil followed by deltamethrin led to a slight but non-significant (P = 0.59) increase in mortality relative to exposure to deltamethrin alone (64.5% versus 69.2%). Similarly, pre-exposure to EN 16-5 yielded a non-significant increase in mortality (to 76.6%; P = 0.85) but a significant increase in the knock down rate (from 48.3% to 78.7%; P < 0.01). Pre-exposure with PBO caused a significant increase in mortality (to 93.1%; P < 0.001) and knockdown rate (100%; P < 0.001), which related to a 2.9 fold decrease in the resistance level. The results provide evidence that metabolic resistance mechanisms are present within the assessed mosquito population. The decrease in time to knock down of this population with deltamethrin following exposure to EN16-5 and PBO is of particular relevance to vector control, where quick knock down is a highly desired characteristic. The suspected resistance mechanisms present in this population merit further investigation through biochemical and molecular analyses for full resistance profile characterization. Bioassays with synergists can provide a quick and easy basis for initial characterization of resistant mosquito populations, without the need of preserved specimens, expensive equipment and substrates or specialized expertise. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:108 / 111
页数:4
相关论文
共 21 条
[1]   Distribution of ace-1R and resistance to carbamates and organophosphates in Anopheles gambiae s.s. populations from Cote d'Ivoire [J].
Alou, Ludovic P. Ahoua ;
Koffi, Alphonsine A. ;
Adja, Maurice A. ;
Tia, Emmanuel ;
Kouassi, Philippe K. ;
Kone, Moussa ;
Chandre, Fabrice .
MALARIA JOURNAL, 2010, 9
[2]  
[Anonymous], WHOCDSCPCMAL9812 PVC
[3]   kdr:: can a single mutation produce an entire insecticide resistance phenotype? [J].
Brooke, Basil D. .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2008, 102 (06) :524-525
[4]   Bioassay and biochemical analyses of insecticide resistance in southern African Anopheles funestus (Diptera: Culicidae) [J].
Brooke, BD ;
Kloke, G ;
Hunt, RH ;
Koekemoer, LL ;
Temu, EA ;
Taylor, ME ;
Small, G ;
Hemingway, J ;
Coetzee, M .
BULLETIN OF ENTOMOLOGICAL RESEARCH, 2001, 91 (04) :265-272
[5]   Interaction of pesticides with p-glycoprotein and other ABC proteins: A survey of the possible importance to insecticide, herbicide and fungicide resistance [J].
Buss, D. S. ;
Callaghan, A. .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2008, 90 (03) :141-153
[6]   Increase in susceptibility to insecticides with aging of wild Anopheles gambiae mosquitoes from Cote d'Ivoire [J].
Chouaibou, Mouhamadou S. ;
Chabi, Joseph ;
Bingham, Georgina V. ;
Knox, Tessa B. ;
N'Dri, Louis ;
Kesse, Nestor B. ;
Bonfoh, Bassirou ;
Jamet, Helen V. Pates .
BMC INFECTIOUS DISEASES, 2012, 12
[7]   Role of cytochrome P450s in insecticide resistance: impact on the control of mosquito-borne diseases and use of insecticides on Earth [J].
David, Jean-Philippe ;
Ismail, Hanafy Mahmoud ;
Chandor-Proust, Alexia ;
Paine, Mark John Ingraham .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2013, 368 (1612)
[8]   The role of agricultural use of insecticides in resistance to pyrethroids in Anopheles gambiae SL in Burkina Faso [J].
Diabate, A ;
Baldet, T ;
Chandre, F ;
Akogbeto, M ;
Guiguemde, TR ;
Darriet, F ;
Brengues, C ;
Guillet, P ;
Hemingway, J ;
Small, GJ ;
Hougard, JM .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2002, 67 (06) :617-622
[9]  
Edi V.A., 2012, EMERG INFECT DIS, V18
[10]   Insect P450 enzymes [J].
Feyereisen, R .
ANNUAL REVIEW OF ENTOMOLOGY, 1999, 44 :507-533