The softness of tumour-cell-derived microparticles regulates their drug-delivery efficiency

被引：179
作者：
Liang, Qingle [1 ]
Bie, Nana [1 ]
Yong, Tuying [1 ]
Tang, Ke [2 ]
Shi, Xiaolong [3 ,4 ]
Wei, Zhaohan [1 ]
Jia, Haibo [5 ]
Zhang, Xiaoqiong [1 ]
Zhao, Haiyan [4 ]
Huang, Wei [1 ]
Gan, Lu [1 ,6 ]
Huang, Bo [2 ,7 ,8 ]
Yang, Xiangliang [1 ,6 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Natl Engn Res Ctr Nanomed, Wuhan, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Biochem & Mol Biol, Wuhan, Hubei, Peoples R China
[3] Guangzhou Univ, Inst Comp Sci & Technol, Guangzhou, Guangdong, Peoples R China
[4] Huazhong Univ Sci & Technol, Sch Artificial Intelligent & Automat, Wuhan, Hubei, Peoples R China
[5] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan, Hubei, Peoples R China
[6] Huazhong Univ Sci & Technol, Hubei Key Lab Bioinorgan Chem & Mat Med, Wuhan, Hubei, Peoples R China
[7] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Dept Immunol, Beijing, Peoples R China
[8] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Key Lab Med Mol Biol, Beijing, Peoples R China
来源：
NATURE BIOMEDICAL ENGINEERING | 2019年 / 3卷 / 09期
基金：
中国国家自然科学基金;
关键词：
EXTRACELLULAR VESICLES; REPOPULATING CELLS; ENDOTHELIAL-CELLS; SYSTEMIC TOXICITY; SIDE-POPULATION; CANCER; HYPOXIA; HETEROGENEITY; ACCUMULATION; PHAGOCYTOSIS;
D O I：
10.1038/s41551-019-0405-4
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Extracellular microparticles (MPs) can function as drug-delivery vehicles for anticancer drugs. Here, we show that the softness of MPs derived from tumour-repopulating cells (TRCs) isolated from three-dimensional fibrin gels enhances the MPs' drug-delivery efficiency. We found that, compared with MPs derived from tumour cells cultured in conventional tissue-culture plastic, TRC-derived MPs intravenously injected in tumour-xenograft-bearing mice showed enhanced accumulation in tumour tissues, enhanced blood-vessel crossing and penetration into tumour parenchyma, and preferential uptake by highly tumorigenic TRCs. We also show that the cytoskeleton-related protein cytospin-A plays a critical role in the regulation of TRC-derived MP softness. The modulation of the mechanical properties of TRC-derived MPs could aid the efficiency of delivery of anticancer drugs.
引用
收藏
页码：729 / 740
页数：12
相关论文
共 47 条
  • [1] Impact of particle elasticity on particle-based drug delivery systems
    Anselmo, Aaron C.
    Mitragotri, Samir
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2017, 108 : 51 - 67
  • [2] Elasticity of Nanopartides Influences Their Blood Circulation, Phagocytosis, Endocytosis, and Targeting
    Anselmo, Aaron C.
    Zhang, Mengwen
    Kumar, Sunny
    Vogus, Douglas R.
    Menegatti, Stefano
    Helgeson, Matthew E.
    Mitragotri, Samir
    [J]. ACS NANO, 2015, 9 (03) : 3169 - 3177
  • [3] Hypoxia-induced dedifferentiation of tumor cells -: A mechanism behind heterogeneity and aggressiveness of solid tumors
    Axelson, H
    Fredlund, E
    Ovenberger, M
    Landberg, G
    Påhlman, S
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2005, 16 (4-5) : 554 - 563
  • [4] Cabral H, 2011, NAT NANOTECHNOL, V6, P815, DOI [10.1038/nnano.2011.166, 10.1038/NNANO.2011.166]
  • [5] Efficient extravasation of tumor-repopulating cells depends on cell deformability
    Chen, Junjian
    Zhou, Wenwen
    Jia, Qiong
    Chen, Junwei
    Zhang, Shuang
    Yao, Wenting
    Wei, Fuxiang
    Zhang, Yuejin
    Yang, Fang
    Huang, Wei
    Zhang, Yao
    Zhang, Huafeng
    Zhang, Yi
    Huang, Bo
    Zhang, Zhihong
    Jia, Haibo
    Wang, Ning
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [6] INHIBITION OF ACTIN POLYMERIZATION BY LATRUNCULIN-A
    COUE, M
    BRENNER, SL
    SPECTOR, I
    KORN, ED
    [J]. FEBS LETTERS, 1987, 213 (02) : 316 - 318
  • [7] Growth Factors, Matrices, and Forces Combine and Control Stem Cells
    Discher, Dennis E.
    Mooney, David J.
    Zandstra, Peter W.
    [J]. SCIENCE, 2009, 324 (5935) : 1673 - 1677
  • [8] Extracellular Vesicles as Novel Delivery Tools for Cancer Treatment
    Erkan, Erdogan Pekcan
    Saydam, Okay
    [J]. CURRENT CANCER DRUG TARGETS, 2016, 16 (01) : 34 - 42
  • [9] Novel approaches for targeted cancer therapy
    Guillemard, V
    Saragovi, HU
    [J]. CURRENT CANCER DRUG TARGETS, 2004, 4 (04) : 313 - 326
  • [10] Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters
    Gyoergy, Bence
    Modos, Karoly
    Pallinger, Eva
    Paloczi, Krisztina
    Pasztoi, Maria
    Misjak, Petra
    Deli, Maria A.
    Sipos, Aron
    Szalai, Aniko
    Voszka, Istvan
    Polgar, Anna
    Toth, Kalman
    Csete, Maria
    Nagy, Gyoergy
    Gay, Steffen
    Falus, Andras
    Kittel, Agnes
    Buzas, Edit I.
    [J]. BLOOD, 2011, 117 (04) : E39 - E48
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