The softness of tumour-cell-derived microparticles regulates their drug-delivery efficiency

被引：185

作者：

Liang, Qingle ^{[1
]}

Bie, Nana ^{[1
]}

Yong, Tuying ^{[1
]}

Tang, Ke ^{[2
]}

Shi, Xiaolong ^{[3
,4
]}

Wei, Zhaohan ^{[1
]}

Jia, Haibo ^{[5
]}

Zhang, Xiaoqiong ^{[1
]}

Zhao, Haiyan ^{[4
]}

Huang, Wei ^{[1
]}

Gan, Lu ^{[1
,6
]}

Huang, Bo ^{[2
,7
,8
]}

Yang, Xiangliang ^{[1
,6
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Natl Engn Res Ctr Nanomed, Wuhan, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Biochem & Mol Biol, Wuhan, Hubei, Peoples R China

[3] Guangzhou Univ, Inst Comp Sci & Technol, Guangzhou, Guangdong, Peoples R China

[4] Huazhong Univ Sci & Technol, Sch Artificial Intelligent & Automat, Wuhan, Hubei, Peoples R China

[5] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan, Hubei, Peoples R China

[6] Huazhong Univ Sci & Technol, Hubei Key Lab Bioinorgan Chem & Mat Med, Wuhan, Hubei, Peoples R China

[7] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Dept Immunol, Beijing, Peoples R China

[8] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Key Lab Med Mol Biol, Beijing, Peoples R China

来源：

NATURE BIOMEDICAL ENGINEERING | 2019年 / 3卷 / 09期

基金：

中国国家自然科学基金;

关键词：

EXTRACELLULAR VESICLES; REPOPULATING CELLS; ENDOTHELIAL-CELLS; SYSTEMIC TOXICITY; SIDE-POPULATION; CANCER; HYPOXIA; HETEROGENEITY; ACCUMULATION; PHAGOCYTOSIS;

D O I：

10.1038/s41551-019-0405-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Extracellular microparticles (MPs) can function as drug-delivery vehicles for anticancer drugs. Here, we show that the softness of MPs derived from tumour-repopulating cells (TRCs) isolated from three-dimensional fibrin gels enhances the MPs' drug-delivery efficiency. We found that, compared with MPs derived from tumour cells cultured in conventional tissue-culture plastic, TRC-derived MPs intravenously injected in tumour-xenograft-bearing mice showed enhanced accumulation in tumour tissues, enhanced blood-vessel crossing and penetration into tumour parenchyma, and preferential uptake by highly tumorigenic TRCs. We also show that the cytoskeleton-related protein cytospin-A plays a critical role in the regulation of TRC-derived MP softness. The modulation of the mechanical properties of TRC-derived MPs could aid the efficiency of delivery of anticancer drugs.

引用

页码：729 / 740

页数：12

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