MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B

被引:14
作者
Chen, Xi [1 ]
Zhang, Lei [2 ]
Geng, JingBo [1 ]
Chen, Zhong [2 ]
Cui, XiaoPeng [1 ]
机构
[1] Nantong Univ, Dept Gastrointestinal Surg, Affiliated Hosp, Nantong, Jiangsu, Peoples R China
[2] Nantong Univ, Dept Gen Surg, Affiliated Hosp, Nantong, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
PROSTATE-CANCER; UP-REGULATION; PROLIFERATION; PROGRESSION; EXPRESSION; GENOMICS; CORONARY; MYC;
D O I
10.1155/2022/8267891
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
MicroRNAs (miRNAs) participate in the formation of multiple diseases, including gastric cancer (GC), through modulating specific targets. Here, we explored the functions and regulatory mechanisms of miR-205-5p in GC. MiR-205-5p levels were detected in GC cells through qRT-PCR. Besides, the role of miR-205-5p in cell proliferation, cell apoptosis, cell cycle, cell invasion, and metastasis was assessed through CCK-8 assay, colony formation, flow cytometry, scratch assay, transwell, and western blot. Moreover, the Starbase website was used to predict the target gene of miR-205-5p, further verified by a dual-luciferase reporter assay. Furthermore, the functional effects of the family with sequence similarity 84 member B (FAM84B) on GC mediated by miR-205-5p upregulation were further investigated. MiR-205-5p expression was decreased in GC cells. Upregulation of miR-205-5p inhibited cell proliferation and metastasis and induced apoptosis and cycle arrest of GC cells. Moreover, FAM84B was predicted and confirmed as a target of miR-205-5p and negatively related to miR-205-5p. Mechanically, FAM84B overexpression partially rescued the functional effects of miR-205-5p upregulation on GC cell progression. This study suggests the potential of miR-205-5p/FAM84B as novel targets for the treatment of GC.
引用
收藏
页数:10
相关论文
共 40 条
[1]   Global surveillance of cancer survival 1995-2009: analysis of individual data for 25 676 887 patients from 279 population-based registries in 67 countries (CONCORD-2) [J].
Allemani, Claudia ;
Weir, Hannah K. ;
Carreira, Helena ;
Harewood, Rhea ;
Spika, Devon ;
Wang, Xiao-Si ;
Bannon, Finian ;
Ahn, Jane V. ;
Johnson, Christopher J. ;
Bonaventure, Audrey ;
Marcos-Gragera, Rafael ;
Stiller, Charles ;
Azevedo e Silva, Gulnar ;
Chen, Wan-Qing ;
Ogunbiyi, Olufemi J. ;
Rachet, Bernard ;
Soeberg, Matthew J. ;
You, Hui ;
Matsuda, Tomohiro ;
Bielska-Lasota, Magdalena ;
Storm, Hans ;
Tucker, Thomas C. ;
Coleman, Michel P. .
LANCET, 2015, 385 (9972) :977-1010
[2]   A common variant associated with prostate cancer in European and African populations [J].
Amundadottir, Laufey T. ;
Sulem, Patrick ;
Gudmundsson, Julius ;
Helgason, Agnar ;
Baker, Adam ;
Agnarsson, Bjarni A. ;
Sigurdsson, Asgeir ;
Benediktsdottir, Kristrun R. ;
Cazier, Jean-Baptiste ;
Sainz, Jesus ;
Jakobsdottir, Margret ;
Kostic, Jelena ;
Magnusdottir, Droplaug N. ;
Ghosh, Shyamali ;
Agnarsson, Kari ;
Birgisdottir, Birgitta ;
Le Roux, Louise ;
Olafsdottir, Adalheidur ;
Blondal, Thorarinn ;
Andresdottir, Margret ;
Gretarsdottir, Olafia Svandis ;
Bergthorsson, Jon T. ;
Gudbjartsson, Daniel ;
Gylfason, Arnaldur ;
Thorleifsson, Gudmar ;
Manolescu, Andrei ;
Kristjansson, Kristleifur ;
Geirsson, Gudmundur ;
Isaksson, Helgi ;
Douglas, Julie ;
Johansson, Jan-Erik ;
Balter, Katarina ;
Wiklund, Fredrik ;
Montie, James E. ;
Yu, Xiaoying ;
Suarez, Brian K. ;
Ober, Carole ;
Cooney, Kathleen A. ;
Gronberg, Henrik ;
Catalona, William J. ;
Einarsson, Gudmundur V. ;
Barkardottir, Rosa B. ;
Gulcher, Jeffrey R. ;
Kong, Augustine ;
Thorsteinsdottir, Unnur ;
Stefansson, Kari .
NATURE GENETICS, 2006, 38 (06) :652-658
[3]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[4]   Genetic susceptibility to sporadic ovarian cancer: A systematic review [J].
Braem, M. G. M. ;
Schouten, L. J. ;
Peeters, P. H. M. ;
van den Brandt, P. A. ;
Onland-Moret, N. C. .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2011, 1816 (02) :132-146
[5]   Long non-coding RNA myocardial infarction associated transcript promotes the proliferation of cholangiocarcinoma cells by targeting miR-551b-3p/CCND1 axis [J].
Chang Weiping ;
Wang Yuan ;
Li WenZhi ;
Geng Zhimin .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2020, 47 (06) :1067-1075
[6]   Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma [J].
Cheng, Caixia ;
Cui, Heyang ;
Zhang, Ling ;
Jia, Zhiwu ;
Song, Bin ;
Wang, Fang ;
Li, Yaoping ;
Liu, Jing ;
Kong, Pengzhou ;
Shi, Ruyi ;
Bi, Yanghui ;
Yang, Bin ;
Wang, Juan ;
Zhao, Zhenxiang ;
Zhang, Yanyan ;
Hu, Xiaoling ;
Yang, Jie ;
He, Chanting ;
Zhao, Zhiping ;
Wang, Jinfen ;
Xi, Yanfeng ;
Xu, Enwei ;
Li, Guodong ;
Guo, Shiping ;
Chen, Yunqing ;
Yang, Xiaofeng ;
Chen, Xing ;
Liang, Jianfang ;
Guo, Jiansheng ;
Cheng, Xiaolong ;
Wang, Chuangui ;
Zhan, Qimin ;
Cui, Yongping .
GIGASCIENCE, 2016, 5
[7]  
De Cola A, 2019, CELL DEATH DISCOV, V5
[8]   miRNA-192 and-215 activate Wnt/β-catenin signaling pathway in gastric cancer via APC [J].
Deng, Shiqi ;
Zhang, Xiaojing ;
Qin, Ying ;
Chen, Wangchun ;
Fan, Hu ;
Feng, Xianling ;
Wang, Jian ;
Yan, Ruibin ;
Zhao, Yanqiu ;
Cheng, Yulan ;
Wei, Yanjie ;
Fan, Xinmin ;
Ashktorab, Hassan ;
Smoot, Duane ;
Meltzer, Stephen J. ;
Li, Song ;
Li, Kuan ;
Peng, Yin ;
Jin, Zhe .
JOURNAL OF CELLULAR PHYSIOLOGY, 2020, 235 (09) :6218-6229
[9]   Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods [J].
Ferlay, J. ;
Colombet, M. ;
Soerjomataram, I. ;
Mathers, C. ;
Parkin, D. M. ;
Pineros, M. ;
Znaor, A. ;
Bray, F. .
INTERNATIONAL JOURNAL OF CANCER, 2019, 144 (08) :1941-1953
[10]   Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells [J].
He, Xi ;
Song, Shanshan ;
Ayon, Ramon J. ;
Balisterieri, Angela ;
Black, Stephen M. ;
Makino, Ayako ;
Wier, W. Gil ;
Zang, Wei-Jin ;
Yuan, Jason X-J .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2018, 314 (04) :C504-C517