Elucidation of novel budding yeast separase mutants

被引：1

作者：

Shimizu, Yoshihito ^{[1
]}

Nagai, Masayoshi ^{[1
]}

Yeasmin, Akter M. S. T. ^{[2
]}

Koike, Naoki ^{[1
]}

Talukdar, Muhammad Waliullah ^{[2
]}

Ushimaru, Takashi ^{[1
,2
]}

机构：

[1] Shizuoka Univ, Grad Sch Sci & Technol, Shizuoka, Japan

[2] Shizuoka Univ, Grad Sch Sci, Fac Sci, Shizuoka 4228529, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2016年 / 80卷 / 03期

关键词：

DNA repair; MPT5; Pumilio; securin; separase; SACCHAROMYCES-CEREVISIAE; ANAPHASE; MITOSIS; COHESIN; LOCALIZATION; CHECKPOINT; SUBUNIT; KINASE; REPAIR; PHOSPHORYLATION;

D O I：

10.1080/09168451.2015.1101337

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.

引用

页码：473 / 478

页数：6

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