Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM

被引：9
作者：
Los, LI
van der Worp, RJ
van Luyn, MJA
Hooymans, JMM
机构：
[1] Univ Groningen, Univ Hosp, Dept Ophthalmol, NL-9700 RB Groningen, Netherlands
[2] Univ Groningen, Dept Lab Med & Pathol, Sect Med Biol, Groningen, Netherlands
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2004年 / 52卷 / 06期
关键词：
collagen IV; ciliary zonules; immunohistochemistry;
D O I：
10.1369/jhc.4A6267.2004
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.
引用
收藏
页码：789 / 795
页数：7
相关论文
共 24 条
[1]   SYNTHESIS AND MIGRATION OF FUCOSE-LABELED-H-3 GLYCOPROTEINS IN THE CILIARY EPITHELIUM OF THE EYE - EFFECTS OF MICROTUBULE-DISRUPTING DRUGS [J].
BENNETT, G ;
HADDAD, A .
AMERICAN JOURNAL OF ANATOMY, 1986, 177 (04) :441-455
[2]  
BERGER E, 1887, ANATOMIE AUGES
[3]   PROTEOGLYCANS ASSOCIATED WITH THE CILIARY ZONULE OF THE RAT EYE - A HISTOCHEMICAL AND IMMUNOCYTOCHEMICAL STUDY [J].
CHAN, FL ;
CHOI, HL .
HISTOCHEMISTRY AND CELL BIOLOGY, 1995, 104 (05) :369-381
[4]   Hyaluronan and chondroitin sulfate proteoglycans are colocalized to the ciliary zonule of the rat eye: A histochemical and immunocytochemical study [J].
Chan, FL ;
Choi, HL ;
Underhill, CB .
HISTOCHEMISTRY AND CELL BIOLOGY, 1997, 107 (04) :289-301
[5]   Expression of basal lamina protein mRNAs in the early embryonic chick eye [J].
Dong, SC ;
Landfair, J ;
Balasubramani, M ;
Bier, ME ;
Cole, G ;
Halfter, W .
JOURNAL OF COMPARATIVE NEUROLOGY, 2002, 447 (03) :261-273
[6]   THE IMMUNOHISTOCHEMICAL LOCALIZATION OF MICROFIBRIL-ASSOCIATED GLYCOPROTEIN (MAGP) IN ELASTIC AND NONELASTIC TISSUES [J].
GIBSON, MA ;
CLEARY, EG .
IMMUNOLOGY AND CELL BIOLOGY, 1987, 65 :345-356
[7]   MICROFIBRILS, ELASTIC ANCHORING COMPONENTS OF THE EXTRACELLULAR-MATRIX, ARE ASSOCIATED WITH FIBRONECTIN IN THE ZONULE OF ZINN AND AORTA [J].
GOLDFISCHER, S ;
COLTOFFSCHILLER, B ;
GOLDFISCHER, M .
TISSUE & CELL, 1985, 17 (04) :441-450
[8]  
HANNOVER A, 1845, MULLERS ARCH, P467
[9]   Synthesis and structural organization of zonular fibers during development and aging [J].
Hanssen, E ;
Franc, S ;
Garrone, R .
MATRIX BIOLOGY, 2001, 20 (02) :77-85
[10]  
HENDERSONSELLER.A, 1996, MITIGATION ADAPTATIO, V1, P1
123