Alkali extraction and in vitro antioxidant activity of Monascus mycelium polysaccharides

被引:23
作者
Wang, Pengrong [1 ]
Chen, Danfeng [1 ]
Jiang, Donghua [1 ]
Dong, Xiameng [1 ]
Chen, Panpan [1 ]
Lin, Yaoxue [1 ]
机构
[1] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
来源
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE | 2014年 / 51卷 / 07期
关键词
Monascus mycelium polysaccharides; Alkali extraction technology; Response surface methodology; Antioxidant activity; RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION; ACID; DERIVATIVES;
D O I
10.1007/s13197-012-0618-y
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In the present work, alkali extraction technology was used to optimize the extraction of Monascus mycelium polysaccharides for the first time. The extracting parameters of alkali extracted Monascus mycelium polysaccharides were optimized by Box-Behnken design (BBD). The optimum conditions were extraction temperature 49 A degrees C, alkali concentration 7%, solvent/material ratio 23:1 (ml/g) and extraction time 2.3 h with an enhanced yield of 10.1%, compared with the yield 4.76% of hot water extraction, indicating that alkali extraction is a more efficient way. In order to discuss the biological activity of alkali extracted polysaccharides, we compared the in vitro antioxidant activity of alkali extracted polysaccharides (AMP) with hot water extracted polysaccharides (HMP). The result showed that AMP have the similar capability of scavenging both superoxide radical and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical of HMP in vitro. Therefore, alkali extraction technology is not only a high-efficiency way to extract AMP, but also can retain the natural antioxidant activities of AMP, which can be used in pharmaceutical and food industries.
引用
收藏
页码:1251 / 1259
页数:9
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