Effect of calcium phosphate-based fillers on the structure and bonding strength of novel gelatin-alginate bioadhesives

被引:16
作者
Cohen, Benny [1 ]
Panker, Maoz [1 ]
Zuckerman, Eyal [1 ]
Foox, Maytal [1 ]
Zilberman, Meital [1 ]
机构
[1] Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel
关键词
hydroxyapatite; tricalcium phosphate; hard tissue adhesives; Soft tissue adhesives; CROSS-LINKED GELATIN; BONE-CEMENT; POLY(L-GLUTAMIC ACID); BIOLOGICAL GLUE; IN-VITRO; ADHESIVES; RELEASE; MATRIX; GELS;
D O I
10.1177/0885328213509502
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Interest in soft and hard tissue adhesives as alternatives for conventional wound closing and bone fixation applications has increased in recent decades as a result of numerous possible advantages such as better comfort and lower cost. A novel bioadhesive based on the natural polymers GA has recently been developed and studied in our laboratory. Hydroxyapatite and tricalcium phosphate are two bioactive ceramics known for their ability to enhance bone regeneration. In the current study, these two bioactive fillers were incorporated into the bioadhesive at concentrations of 0.125, 0.25 and 0.5% w/v, and their effects on the resulting adherence properties to soft and hard tissues were studied. Porcine skin and cortical portions of bovine femurs were used as soft and hard tissue specimens, respectively. The bonding strength was evaluated using an Instron universal testing machine in tensile mode, and the microstructure analysis was based on environmental scanning electron microscope observations. Both bioactive fillers were found to have a reinforcing effect on the adhesives, significantly improving their adhesion to soft tissues in certain concentrations. The best bonding strength results were obtained for 0.25% hydroxyapatite and 0.5% w/v tricalcium phosphate-18.1 +/- 4.0 and 15.2 +/- 2.6 kPa, respectively, compared with 8.4 +/- 2.3 kPa for adhesive with no fillers. The improved adherence is probably related to the stiffness of the insoluble hydroxyapatite and tricalcium phosphate particles which reinforce the adhesive. These particles can clearly be observed in the environmental scanning electron microscope analysis. The potential of these fillers to increase the bonding strength of the adhesive to hard tissues was also demonstrated. Hydroxyapatite and tricalcium phosphate thus improve our new gelatin-alginate bioadhesives, which can be used for both soft and hard tissue adhesive applications.
引用
收藏
页码:1366 / 1375
页数:10
相关论文
共 36 条
  • [11] Cell attachment and response to photocured, degradable bone adhesives containing tricalcium phosphate and purmorphamine
    Gellynck, Kris
    Abou Neel, Ensanya A.
    Li, HaoYing
    Mardas, Nikos
    Donos, Nikolaos
    Buxton, Paul
    Young, Anne M.
    [J]. ACTA BIOMATERIALIA, 2011, 7 (06) : 2672 - 2677
  • [12] INVIVO HISTOLOGIC AND BIOMECHANICAL CHARACTERIZATION OF A BIODEGRADABLE PARTICULATE COMPOSITE BONE-CEMENT
    GERHART, TN
    RENSHAW, AA
    MILLER, RL
    NOECKER, RJ
    HAYES, WC
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1989, 23 (01): : 1 - 16
  • [13] Flexural and fatigue properties of a bone cement based upon polyethylmethacrylate and hydrxyapatite
    Harper, EJ
    Behiri, JC
    Bonfield, W
    [J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1995, 6 (12) : 799 - 803
  • [14] A NEW SEALANT FOR KNITTED DACRON PROSTHESES - MINIMALLY CROSS-LINKED GELATIN
    JONAS, RA
    ZIEMER, G
    SCHOEN, FJ
    BRITTON, L
    CASTANEDA, AR
    [J]. JOURNAL OF VASCULAR SURGERY, 1988, 7 (03) : 414 - 419
  • [15] Adsorption of immunogamma globulin onto various synthetic calcium hydroxyapatite particles
    Kandori, K
    Miyagawa, K
    Ishikawa, T
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 273 (02) : 406 - 413
  • [16] Kraus R, 2006, EUR J TRAUMA, V32, P141
  • [17] Characterization of the network structure of carbodiimide cross-linked gelatin gels
    Kuijpers, AJ
    Engbers, GHM
    Feijen, J
    De Smedt, SC
    Meyvis, TKL
    Demeester, J
    Krijgsveld, J
    Zaat, SAJ
    Dankert, J
    [J]. MACROMOLECULES, 1999, 32 (10) : 3325 - 3333
  • [18] Lee HB, 2003, POLYM BIOMATERIALS
  • [19] LIEBENDORFER A, 1995, 21 ANN M SOC BIOM SA, P335
  • [20] Evaluation of sodium alginate as drug release modifier in matrix tablets
    Liew, CV
    Chan, LW
    Ching, AL
    Heng, PWS
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 309 (1-2) : 25 - 37