Conserved roles for slit and robo proteins in midline commissural axon guidance

被引:353
作者
Long, H
Sabatier, C
Ma, L
Plump, A
Yuan, WL
Ornitz, DM
Tamada, A
Murakami, F
Goodman, CS
Tessier-Lavigne, M
机构
[1] Stanford Univ, Howard Hughes Med Inst, Dept Biol Sci, Stanford, CA 94305 USA
[2] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Anat, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Biochem & Biophys, San Francisco, CA 94143 USA
[4] Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA
[5] Natl Inst Basic Biol, Div Behav & Neurobiol, Okazaki, Aichi 4448585, Japan
[6] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1016/S0896-6273(04)00179-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In Drosophila, Slit at the midline activates Robo receptors on commissural axons, thereby repelling them out of the midline into distinct longitudinal tracts on the contralateral side of the central nervous system. In the vertebrate spinal cord, Robo1 and Robo2 are expressed by commissural neurons, whereas all three Slit homologs are expressed at the ventral midline. Previous analysis of Slit1;Slit2 double mutant spinal cords failed to reveal a defect in commissural axon guidance. We report here that when all six Slit alleles are removed, many commissural axons fail to leave the midline, while others recross it. In addition, Robo1 and Robo2 single mutants show guidance defects that reveal a role for these two receptors in guiding commissural axons to different positions within the ventral and lateral funiculi. These results demonstrate a key role for Slit/Robo signaling in midline commissural axon guidance in vertebrates.
引用
收藏
页码:213 / 223
页数:11
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