RNF4 negatively regulates NF-κB signaling by down-regulating TAB2

Most of NF-kappa B (nuclear factor kappa B) signaling molecules have various types of post-translational modifications. In this study, we focused on ubiquitination and designed a siRNA library including most ubiquitin-binding domains. With this library, we identified several candidate regulators of canonical NF-kappa B pathway, including RNF4. Overexpression of RNF4 impaired NF-kappa B activation in a dose-dependent manner, whereas RNF4 knockdown potentiated NF-kappa B activation. We showed that RNF4 interacts with the TAK1-TAB2-TAB3 complex, but not TAB1. Further, we found that RNF4 specifically down-regulated TAB2 through a lysosomal pathway, and knockdown of RNF4 impaired endogenous TAB2 degradation. Therefore, our findings will provide new insights into the negative regulation of NF-kappa B signaling. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.