ferredoxin-NADP(+)-oxidoreductase;
Arabidopsis;
photosynthesis;
electron transfer;
structural model;
chloroplast;
D O I:
10.1111/j.1365-313X.2006.03014.x
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
In Arabidopsis thaliana, the chloroplast-targeted enzyme ferredoxin-NADP(+)-oxidoreductase (FNR) exists as two isoforms, AtLFNR1 and AtLFNR2, encoded by the genes At5g66190 and At1g20020, respectively. Both isoforms are evenly distributed between the thylakoids and soluble stroma, and they are separated by two-dimensional electrophoresis in four distinct spots, suggesting post-translational modification of both isoforms. To reveal the functional specificity of AtLFNR1, we have characterized the T-DNA insertion mutants with an interrupted At5g66190 gene. Absence of AtLFNR1 resulted in a reduced size of the rosette with pale green leaves, which was accompanied by a low content of chlorophyll and light-harvesting complex proteins. Also the photosystem I/photosystem II (PSI/PSII) ratio was significantly lower in the mutant, but the PSII activity, measured as the F-V/F-M ratio, remained nearly unchanged and the excitation pressure of PSII was lower in the mutants than in the wild type. A slow re-reduction rate of P700 measured in the mutant plants suggested that AtLFNR1 is involved in PSI-dependent cyclic electron flow. Impaired function of FNR also resulted in decreased capacity for carbon fixation, whereas nitrogen metabolism was upregulated. In the absence of AtLFNR1, we found AtLFNR2 exclusively in the stroma, suggesting that AtLFNR1 is required for membrane attachment of FNR. Structural modeling supports the formation of a AtLFNR1-AtLFNR2 heterodimer that would mediate the membrane attachment of AtLFNR2. Dimer formation, in turn, might regulate the distribution of electrons between the cyclic and linear electron transfer pathways according to environmental cues.
机构:
Schepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Massachusetts Eye & Ear Infirm, Boston, MA 02114 USA
Harvard Univ, Harvard PhD Program Virol, Div Med Sci, Boston, MA 02115 USA
Harvard Med Sch, Ocular Genom Inst, Dept Ophthalmol, Boston, MA USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Dudek, Amanda M.
Zinn, Eric M.
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机构:
Schepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Massachusetts Eye & Ear Infirm, Boston, MA 02114 USA
Harvard Univ, Harvard PhD Program Virol, Div Med Sci, Boston, MA 02115 USA
Harvard Med Sch, Ocular Genom Inst, Dept Ophthalmol, Boston, MA USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Zinn, Eric M.
Pillay, Sirika
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机构:
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Pillay, Sirika
Puschnik, Andreas S.
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机构:
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Puschnik, Andreas S.
Nagamine, Claude M.
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机构:
Stanford Univ, Dept Comparat Med, Sch Med, Stanford, CA 94305 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Nagamine, Claude M.
Cheng, Fang
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机构:
Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Cheng, Fang
Qiu, Jianming
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h-index: 0
机构:
Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66103 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Qiu, Jianming
Carette, Jan E.
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Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Carette, Jan E.
Vandenberghe, Luk H.
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机构:
Schepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
Massachusetts Eye & Ear Infirm, Boston, MA 02114 USA
Harvard Med Sch, Ocular Genom Inst, Dept Ophthalmol, Boston, MA USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USASchepens Eye Res Inst, Grousbeck Gene Therapy Ctr, Boston, MA USA
机构:
Hosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Zabinyakov, Nikita
Bullivant, Garrett
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机构:
Univ Toronto, Div Clin & Metab Genet, Dept Pediat, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Bullivant, Garrett
Cao, Feng
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机构:
Hosp Sick Children, Res Inst, Neurosci & Mental Hlth Program, Toronto, ON, Canada
Univ Toronto, Dept Physiol, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Cao, Feng
Ojeda, Matilde Fernandez
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h-index: 0
机构:
Vrije Univ Amsterdam, Med Ctr, Dept Clin Chem, Metab Lab,Neurosci Amsterdam, Amsterdam, NetherlandsHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Ojeda, Matilde Fernandez
Jia, Zheng Ping
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Sick Children, Res Inst, Neurosci & Mental Hlth Program, Toronto, ON, Canada
Univ Toronto, Dept Physiol, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Jia, Zheng Ping
Wen, Xiao-Yan
论文数: 0引用数: 0
h-index: 0
机构:
St Michaels Hosp, Zebrafish Ctr Adv Drug Discovery, Toronto, ON, Canada
St Michaels Hosp, Li Ka Shing Knowledge Inst, Keenan Res Ctr Biomed Sci, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Wen, Xiao-Yan
Dowling, James J.
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机构:
Hosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Univ Toronto, Div Neurol, Dept Pediat, Toronto, ON, Canada
Univ Toronto, Dept Mol Genet, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Dowling, James J.
Salomons, Gajja S.
论文数: 0引用数: 0
h-index: 0
机构:
Vrije Univ Amsterdam, Med Ctr, Dept Clin Chem, Metab Lab,Neurosci Amsterdam, Amsterdam, Netherlands
Neurosci Campus, Amsterdam, NetherlandsHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Salomons, Gajja S.
Mercimek-Andrews, Saadet
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada
Univ Toronto, Div Clin & Metab Genet, Dept Pediat, Toronto, ON, Canada
Univ Toronto, Inst Med Sci, Toronto, ON, CanadaHosp Sick Children, Res Inst, Genet & Genome Biol Program, Toronto, ON, Canada