Polymer recycling in aqueous two-phase extractions using thermoseparating ethylene oxide-propylene oxide copolymers

被引:71
作者
Persson, J [1 ]
Kaul, A [1 ]
Tjerneld, F [1 ]
机构
[1] Univ Lund, Dept Biochem, S-22100 Lund, Sweden
来源
JOURNAL OF CHROMATOGRAPHY B | 2000年 / 743卷 / 1-2期
关键词
partitioning; aqueous two-phase systems; polymer recycling; ethylene oxide-propylene oxide; thermoseparating polymers;
D O I
10.1016/S0378-4347(00)00213-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This is a study on the recovery and recycling of copolymer in aqueous two-phase systems containing random copolymers of ethylene oxide (EO) and propylene oxide (PO). The random copolymers separate from water solution when heated above the lower critical solution temperature (LCST). The primary phase systems were composed of EOPO copolymer and hydroxypropyl or hydroxyethyl starch. After phase separation the upper EOPO phase was removed and subjected to temperature induced phase separation. Copolymers with different EO/PO compositions have been investigated, EO50PO50 [50% EO and 50% PO (w/w)], EO30PO70 and EO20PO80. The temperature required for thermoseparation decreases when the PO content of the copolymer is increased. The effect on the recovery of copolymer after addition of salts, a second polymer or protein was investigated The added components increased the recovery of copolymer after thermoseparation, e.g., increased the amount copolymer separated from the water phase after thermoseparation. Recycling of copolymer and measurements of polymer concentrations in the primary top and bottom phases after repeated recycling steps was performed. The fluctuation in polymer concentration of the phases was very small after recycling up to four times. Partitioning of the proteins BSA and lysozyme was studied in primary phase systems after recycling of copolymer. The partition coefficients of total protein and lysozyme was not significantly changed during recycling of copolymer. More than 90% of the copolymer could be recovered in the thermoseparation step by optimising the temperature and time for thermoseparation. In repeated phase partitionings in EOPO-starch systems the EO50PO50 copolymer could be recovered to 77% including losses in primary system and thermoseparation, which is equivalent to a total copolymer reuse of 4.3 times. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:115 / 126
页数:12
相关论文
共 27 条
[1]   APPLICATION OF TEMPERATURE-INDUCED PHASE PARTITIONING AT AMBIENT-TEMPERATURE FOR ENZYME-PURIFICATION [J].
ALRED, PA ;
KOZLOWSKI, A ;
HARRIS, JM ;
TJERNELD, F .
JOURNAL OF CHROMATOGRAPHY A, 1994, 659 (02) :289-298
[2]   Effects of salts and the surface hydrophobicity of proteins on partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide-propylene oxide copolymers [J].
Berggren, K ;
Johansson, HO ;
Tjerneld, F .
JOURNAL OF CHROMATOGRAPHY A, 1995, 718 (01) :67-79
[3]   EXTRACTIVE PURIFICATION OF ENZYMES FROM ANIMAL TISSUE USING AQUEOUS 2 PHASE SYSTEMS - PILOT SCALE STUDIES [J].
BOLAND, MJ ;
HESSELINK, PGM ;
PAPAMICHAEL, N ;
HUSTEDT, H .
JOURNAL OF BIOTECHNOLOGY, 1991, 19 (01) :19-33
[4]  
DECKER LA, 1977, WORTHINGTON ENZYME M, P185
[5]   ON THE THEORY OF LOWER CRITICAL SOLUTION POINTS IN HYDROGEN-BONDED MIXTURES [J].
GOLDSTEIN, RE .
JOURNAL OF CHEMICAL PHYSICS, 1984, 80 (10) :5340-5341
[6]  
Harris P A, 1991, Bioseparation, V2, P237
[7]  
HUSTEDT H, 1985, PARTITIONING AQUEOUS, P529
[8]   EFFECTS OF SALTS ON PARTITION OF PROTEINS IN AQUEOUS POLYMERIC BIPHASIC SYSTEMS [J].
JOHANSSON, G .
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY, 1974, B 28 (08) :873-882
[9]  
Johansson HO, 1995, BIOSEPARATION, V5, P269
[10]   EXPERIMENTAL AND THEORETICAL-STUDY OF PHASE-SEPARATION IN AQUEOUS-SOLUTIONS OF CLOUDING POLYMERS AND CARBOXYLIC-ACIDS [J].
JOHANSSON, HO ;
KARLSTROM, G ;
TJERNELD, F .
MACROMOLECULES, 1993, 26 (17) :4478-4483