Virus replication and cytokine production in dengue virus-infected human B lymphocytes

被引:75
作者
Lin, YW
Wang, KJ
Lei, HY
Lin, YS
Yeh, TM
Liu, HS
Liu, CC
Chen, SH [1 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Coll Med, Dept Med Technol, Tainan 70101, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Dept Pediat, Tainan 70101, Taiwan
关键词
D O I
10.1128/JVI.76.23.12242-12249.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that DV actively replicated in B cells. Virus infection also induced B cells to produce interleukin-6 and tumor necrosis factor alpha, which have been previously implicated in virus pathogenesis. In addition, a heterologous antibody was able to enhance both virus and cytokine production in B cells. Furthermore, the levels of virus replication, antibody-enhanced virus replication, and cytokine responses observed in B cells were not statistically different from those in monocytes. These results suggest that B cells may play an important role in DV pathogenesis.
引用
收藏
页码:12242 / 12249
页数:8
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