Novel serum microRNAs panel on the diagnostic and prognostic implications of hepatocellular carcinoma

被引：25
作者：
An, Yang [1 ]
Gao, Song [2 ]
Zhao, Wen-Chao [1 ]
Qiu, Bao-An [1 ]
Xia, Nian-Xin [1 ]
Zhang, Peng-Jun [2 ]
Fan, Zhen-Ping [3 ]
机构：
[1] Navy Gen Hosp Chinese Peoples Liberat Army, Dept Hepatobiliarypancreat Surgey, Beijing 100048, Peoples R China
[2] Peking Univ, Canc Hosp & Inst, Intervent Therapy Dept, Minist Educ Beijing,Key Lab Carcinogenesis & Tran, Beijing 100142, Peoples R China
[3] Beijing 302 Mil Hosp, Liver Dis Ctr Cadre Med Care, Xi Si Huan Zhong Rd 100, Beijing 100039, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2018年 / 24卷 / 24期
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
Hepatocellular carcinoma; MicroRNAs; Serum; Biomarker; Diagnosis; CIRCULATING MICRORNAS; EXPRESSION PROFILE; LIVER-CANCER; BIOMARKERS; PCR; CONTRIBUTES;
D O I：
10.3748/wjg.v24.i24.2596
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM To determine a panel of serum microRNAs (miRNAs) that could be used as novel biomarkers for diagnosis of hepatocellular carcinoma (HCC). METHODS We initially screened 9 out of 754 serum miRNAs by TaqMan Low Density Array in two pooled samples respectively from 35 HCC and 35 normal controls, and then validated individually by RT-qPCR in another 114 patients and 114 controls arranged in two phases. The changes of the selected miRNAs after operation and their prognostic value were examined. RESULTS miR-375, miR-10a, miR-122 and miR-423 were found to be significantly higher in HCC than in controls (P < 0.0001), and the area under the receiver-operating-characteristic curve for the 4-miRNA panel was 0.995 (95%CI: 0.985-1). All the four miRNAs were significantly reduced after surgical removal of the tumors (P < 0.0001), while still higher than normal controls (at least P < 0.05) CONCLUSION The four serum miRNAs (miR-375, miR-10a, miR-122 and miR-423) could potentially serve as novel biomarkers for the diagnostic and prognostic of HCC.
引用
收藏
页码：2596 / 2604
页数：9
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