Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress

Present work focuses on tissue and concentration-dependent effect of nitric oxide (NO) on the modulation of cytosolic peroxidase (POD; EC 1.11.1.7) activity in 2-day old etiolated sunflower (Helianthus annuus L.) seedlings. Exogenously supplied NO (in the form of sodium nitroprusside [SNP] or diethylenetriamine NONOate [DETA]; 125 to 500 mu M) results in noteworthy enhancement in seedling growth in a concentration dependent manner irrespective of salt-stress and differentially affects POD activity in 2-day old seedling cotyledons. Elevated NO availability leads to an increase in the specific activity of POD in a concentration-dependent manner within 48 hrs as a rapid signaling response. Purification of POD protein using immunoprecipitation technique has shown that cotyledons derived from salt stressed seedlings exhibit a higher extent of tyrosine nitration of POD as compared to the control seedlings. Out of the four tyrosine residues found in the amino acid sequence of POD, the one at position 100 has been predicted to undergo nitration. Thus, a probable NO-POD crosstalk is evident in sunflower seedling cotyledons accompanying salt stress.