Trehalose is a potent PCR enhancer:: Lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose

被引:84
作者
Spiess, AN [1 ]
Mueller, N [1 ]
Ivell, R [1 ]
机构
[1] Ctr Innovat Med, Inst Hormone & Fertil Res, D-20251 Hamburg, Germany
关键词
D O I
10.1373/clinchem.2004.031336
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
[No abstract available]
引用
收藏
页码:1256 / 1259
页数:4
相关论文
共 28 条
[21]   Thermotolerance in Saccharomyces cerevisiae:: the Yin and Yang of trehalose [J].
Singer, MA ;
Lindquist, S .
TRENDS IN BIOTECHNOLOGY, 1998, 16 (11) :460-468
[22]   Bacterial osmoadaptation: the role of osmolytes in bacterial stress and virulence [J].
Sleator, RD ;
Hill, C .
FEMS MICROBIOLOGY REVIEWS, 2002, 26 (01) :49-71
[23]   SPEER -: A new family of testis-specific genes from the mouse [J].
Spiess, AN ;
Walther, N ;
Müller, N ;
Balvers, M ;
Hansis, C ;
Ivell, R .
BIOLOGY OF REPRODUCTION, 2003, 68 (06) :2044-2054
[24]   A highly efficient method for long-chain cDNA synthesis using trehalose and betaine [J].
Spiess, AN ;
Ivell, R .
ANALYTICAL BIOCHEMISTRY, 2002, 301 (02) :168-174
[25]   DENATURANTS OR COSOLVENTS IMPROVE THE SPECIFICITY OF PCR AMPLIFICATION OF A G+C-RICH DNA USING GENETICALLY-ENGINEERED DNA-POLYMERASES [J].
VARADARAJ, K ;
SKINNER, DM .
GENE, 1994, 140 (01) :1-5
[26]   Template secondary structure promotes polymerase jumping during PCR amplification [J].
Viswanathan, VK ;
Krcmarik, K ;
Cianciotto, NP .
BIOTECHNIQUES, 1999, 27 (03) :508-511
[27]  
Weissensteiner T, 1996, BIOTECHNIQUES, V21, P1102
[28]  
Weissensteiner T, 1998, NUCLEIC ACIDS RES, V26, P687