Trehalose is a potent PCR enhancer:: Lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose

被引：84

作者：

Spiess, AN ^{[1
]}

Mueller, N ^{[1
]}

Ivell, R ^{[1
]}

机构：

[1] Ctr Innovat Med, Inst Hormone & Fertil Res, D-20251 Hamburg, Germany

来源：

CLINICAL CHEMISTRY | 2004年 / 50卷 / 07期

关键词：

D O I：

10.1373/clinchem.2004.031336

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

[No abstract available]

引用

页码：1256 / 1259

页数：4

共 28 条

[1] Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content [J].

Baskaran, N ;

Kandpal, RP ;

Bhargava, AK ;

Glynn, MW ;

Bale, A ;

Weissman, SM .

GENOME RESEARCH, 1996, 6 (07) :633-638

[2] OXYTOCIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID - CHARACTERIZATION, REGULATION, AND CELLULAR-LOCALIZATION IN THE RAT PITUITARY-GLAND [J].

BRETON, C ;

PECHOUX, C ;

MOREL, G ;

ZINGG, HH .

ENDOCRINOLOGY, 1995, 136 (07) :2928-2936

[3] Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA [J].

Carninci, P ;

Nishiyama, Y ;

Westover, A ;

Itoh, M ;

Nagaoka, S ;

Sasaki, N ;

Okazaki, Y ;

Muramatsu, M ;

Hayashizaki, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (02) :520-524

[4] Novel sulfoxides facilitate GC-rich template amplification [J].

Chakrabarti, R ;

Schutt, CE .

BIOTECHNIQUES, 2002, 32 (04) :866-+

[5]

DEMEKE T, 1992, BIOTECHNIQUES, V12, P332

[6] New insights on trehalose: a multifunctional molecule [J].

Elbein, AD ;

Pan, YT ;

Pastuszak, I ;

Carroll, D .

GLYCOBIOLOGY, 2003, 13 (04) :17R-27R

[7]

Frackman S., 1998, PROMEGA NOTES, V65, P27

[8]

Henke W, 1998, NUCLEIC ACIDS RES, V26, P687

[9] Betaine improves the PCR amplification of GC-rich DNA sequences [J].

Henke, W ;

Herdel, K ;

Jung, K ;

Schnorr, D ;

Loening, SA .

NUCLEIC ACIDS RESEARCH, 1997, 25 (19) :3957-3958

[10] Stabilized, freeze-dried PCR mix for detection of mycobacteria [J].

Klatser, PR ;

Kuijper, S ;

van Ingen, CW ;

Kolk, AHJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (06) :1798-1800

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