Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes

被引：72

作者：

Nishizawa, Matsuhiko ^{[1
]}

Nozaki, Hyuma ^{[1
]}

Kaji, Hirokazu ^{[1
]}

Kitazume, Takahiro ^{[1
]}

Kobayashi, Noriyuki ^{[1
]}

Ishibashi, Takeshi ^{[1
]}

Abe, Takashi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Engn, Dept Bioengn & Robot, Sendai, Miyagi 9808579, Japan

来源：

BIOMATERIALS | 2007年 / 28卷 / 08期

关键词：

cardiomyocyte; cell culture; electrical stimulation; electroactive polymer; electrochemistry;

D O I：

10.1016/j.biomaterials.2006.11.034

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The electrically conducting polymer polypyrrole (PPy) was electrochemically deposited onto Pt microelectrodes on a polyimide (PI) substrate. Pre-modification of the PI surface with a self-assembled monolayer of octadecyltrichlorosilane-induced anisotropic lateral growth of PPy along the P1 surface and enhanced adhesive strength of the PPy film. The lateral growth of PPy film around the electrode anchored the whole film to the substrate. External stimulation of cultured cardiac myocytes was carried out using the PPy-coated microelectrode. The myocytes on the microelectrode substrate were electrically conjugated to form a sheet, and showed synchronized beating upon stimulation. The threshold charge for effective stimulation of a 0.8cm(2) sheet of myocytes was around 0.2 mu C, roughly corresponding to a membrane depolarization of 250 mV. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1480 / 1485

页数：6

共 21 条

[1] Microelectrode Array Recordings of Patterned Hippocampal Neurons for Four Weeks [J].

Chang, John C. ;

Brewer, Gregory J. ;

Wheeler, Bruce C. .

BIOMEDICAL MICRODEVICES, 2000, 2 (04) :245-253

[2] Electrochemical deposition and characterization of conducting polymer polypyrrole/PSS on multichannel neural probes [J].

Cui, XY ;

Hetke, JF ;

Wiler, JA ;

Anderson, DJ ;

Martin, DC .

SENSORS AND ACTUATORS A-PHYSICAL, 2001, 93 (01) :8-18

[3] Semiconductor chips with ion channels, nerve cells and brain [J].

Fromherz, P .

PHYSICA E-LOW-DIMENSIONAL SYSTEMS & NANOSTRUCTURES, 2003, 16 (01) :24-34

[4] Fabrication and biocompatibility of polypyrrole implants suitable for neural prosthetics [J].

George, PM ;

Lyckman, AW ;

LaVan, DA ;

Hegde, A ;

Leung, Y ;

Avasare, R ;

Testa, C ;

Alexander, PM ;

Langer, R ;

Sur, M .

BIOMATERIALS, 2005, 26 (17) :3511-3519

[5] Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays [J].

James, CD ;

Davis, R ;

Meyer, M ;

Turner, A ;

Turner, S ;

Withers, G ;

Kam, L ;

Banker, G ;

Craighead, H ;

Isaacson, M ;

Turner, J ;

Shain, W .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2000, 47 (01) :17-21

[6]

Jurgen Breme H, 1998, METALS BIOMATERIALS

[7] Localized chemical stimulation to micropatterned cells using multiple laminar fluid flows [J].

Kaji, H ;

Nishizawa, M ;

Matsue, T .

LAB ON A CHIP, 2003, 3 (03) :208-211

[8] Pharmacological characterization of micropatterned cardiac myocytes [J].

Kaji, H ;

Takii, Y ;

Nishizawa, M ;

Matsue, T .

BIOMATERIALS, 2003, 24 (23) :4239-4244

[9] Stimulation of single isolated adult ventricular myocytes within a low volume using a planar microelectrode array [J].

Klauke, N ;

Smith, GL ;

Cooper, J .

BIOPHYSICAL JOURNAL, 2003, 85 (03) :1766-1774

[10]

MATTBEWS GG, 1991, CELLULAR PHYSL NERVE

← 1 2 3 →