Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea

被引：10

作者：

Chirkov, Sergei ^{[1
]}

Ivanov, Peter ^{[1
]}

Sheveleva, Anna ^{[1
]}

Kudryavtseva, Anna ^{[2
]}

Prikhodko, Yuri ^{[3
]}

Mitrofanova, Irina ^{[4
]}

机构：

[1] Moscow MV Lomonosov State Univ, Moscow, Russia

[2] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia

[3] All Russian Ctr Plant Quarantine, Moscow, Russia

[4] Natl Sci Ctr, Nikita Bot Gardens, Jalta, Russia

来源：

ARCHIVES OF VIROLOGY | 2016年 / 161卷 / 02期

关键词：

Plum pox virus; Strain D; 454; Pyrosequencing; Phylogenetic analysis; Serological analysis; MOLECULAR CHARACTERIZATION; GENETIC DIVERSITY; POTYVIRUS; SHARKA; HOST; PCR;

D O I：

10.1007/s00705-015-2658-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Numerous plum pox virus (PPV) strain D isolates have been found in geographically distant regions of European Russia and the Crimean peninsula on different stone fruit hosts. Phylogenetic analysis of their partial and complete genomes suggests multiple introductions of PPV-D into Russia. Distinct natural isolates from Prunus tomentosa were found to bear unique amino acid substitutions in the N-terminus of the coat protein (CP) that may contribute to the adaptation of PPV-D to this host. Serological analysis using the PPV-D-specific monoclonal antibody 4DG5 provided further evidence that mutations at positions 58 and 59 of the CP are crucial for antibody binding.

引用

页码：425 / 430

页数：6

共 28 条

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