Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo

被引：107

作者：

Qi, Xin ^{[1
]}

Pei, Peng ^{[2
]}

Zhu, Min ^{[2
]}

Du, Xiaoyu ^{[2
]}

Xin, Chen ^{[2
]}

Zhao, Shichang ^{[1
]}

Li, Xiaolin ^{[1
]}

Zhu, Yufang ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthoped Surg, Shanghai, Peoples R China

[2] Univ Shanghai Sci & Technol, Sch Mat Sci & Engn, 516 Jungong Rd, Shanghai 200093, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

COMPOSITE SCAFFOLDS; BIOCERAMIC SCAFFOLDS; DRUG-DELIVERY; CEMENT; DEFECT; CELLS; BIOCOMPATIBILITY; MINERALIZATION; FABRICATION; SUBSTITUTE;

D O I：

10.1038/srep42556

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.

引用

页数：12

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