Label-free multi-photon imaging of dysplasia in Barrett's esophagus

被引：14

作者：

Mehravar, Soroush ^{[1
]}

Banerjee, Bhaskar ^{[1
,2
,3
]}

Chatrath, Hemant ^{[2
]}

Amirsolaimani, Babak ^{[1
]}

Patel, Krunal ^{[2
]}

Patel, Charmi ^{[2
]}

Norwood, Robert A. ^{[1
]}

Peyghambarian, Nasser ^{[1
]}

Kieu, Khanh ^{[1
]}

机构：

[1] Univ Arizona, Coll Opt Sci, 1630 E Univ Blvd, Tucson, AZ 85721 USA

[2] Univ Arizona, Coll Med, 1501 N Campbell Ave, Tucson, AZ 85724 USA

[3] Univ Arizona, Dept Biomed Engn, 1127 E James E Rogers Way, Tucson, AZ 85721 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 01期

关键词：

HARMONIC-GENERATION MICROSCOPY; IN-VIVO; 3RD-HARMONIC GENERATION; RADIOFREQUENCY ABLATION; MOUSE-BRAIN; TISSUE; ADENOCARCINOMA; ENDOSCOPY; DIAGNOSIS; RESECTION;

D O I：

10.1364/BOE.7.000148

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Barrett's esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of back-scattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett's. (C)2015 Optical Society of America

引用

页码：148 / 157

页数：10

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