Hybrid Aptanner-Antibody Linked Fluorescence Resonance Energy Transfer Based Detection of Trinitrotoluene

被引：45
作者：
Sabherwal, Priyanka [1 ]
Shorie, Munish [2 ]
Pathania, Preeti [2 ]
Chaudhary, Shilpa [2 ]
Bhasin, K. K. [3 ]
Bhalla, Vijayender [2 ]
Suri, C. Raman [2 ]
机构：
[1] Inst Nano Sci & Technol, Sect 64, Mohali 160064, India
[2] CSIR Inst Microbial Technol, Sect 39 A, Chandigarh 160036, India
[3] Panjab Univ, Dept Chem, Chandigarh 160014, India
来源：
ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 15期
关键词：
2,4,6-TRINITROTOLUENE TNT; ELECTRONIC DETECTION; SPECTROMETRY; IMMUNOASSAY; SENSOR; FRET;
D O I：
10.1021/ac501388a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
引用
收藏
页码：7200 / 7204
页数：5
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