Hybrid Aptanner-Antibody Linked Fluorescence Resonance Energy Transfer Based Detection of Trinitrotoluene

被引：45

作者：

Sabherwal, Priyanka ^{[1
]}

Shorie, Munish ^{[2
]}

Pathania, Preeti ^{[2
]}

Chaudhary, Shilpa ^{[2
]}

Bhasin, K. K. ^{[3
]}

Bhalla, Vijayender ^{[2
]}

Suri, C. Raman ^{[2
]}

机构：

[1] Inst Nano Sci & Technol, Sect 64, Mohali 160064, India

[2] CSIR Inst Microbial Technol, Sect 39 A, Chandigarh 160036, India

[3] Panjab Univ, Dept Chem, Chandigarh 160014, India

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 15期

关键词：

2,4,6-TRINITROTOLUENE TNT; ELECTRONIC DETECTION; SPECTROMETRY; IMMUNOASSAY; SENSOR; FRET;

D O I：

10.1021/ac501388a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.

引用

页码：7200 / 7204

页数：5

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