Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation

被引：9

作者：

Ferreira, Joshua P. ^{[1
]}

Noderer, William L. ^{[1
]}

de Arce, Alexander J. Diaz ^{[1
]}

Wang, Clifford L. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

来源：

BIOENGINEERED | 2014年 / 5卷 / 03期

关键词：

upstream open reading; frames; uORF; translation initiation; ribosome scanning; cellular stress; posttranscriptional regulation; MESSENGER-RNA TRANSLATION; AUG INITIATOR CODON; EUKARYOTIC TRANSLATION; MAMMALIAN-CELLS; GENE-EXPRESSION; REINITIATION; STRESS; GCN4; EFFICIENCY; NUCLEOTIDES;

D O I：

10.4161/bioe.27607

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.

引用

页码：186 / 192

页数：7

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