Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation

被引：9
作者：
Ferreira, Joshua P. [1 ]
Noderer, William L. [1 ]
de Arce, Alexander J. Diaz [1 ]
Wang, Clifford L. [1 ]
机构：
[1] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA
来源：
BIOENGINEERED | 2014年 / 5卷 / 03期
关键词：
upstream open reading; frames; uORF; translation initiation; ribosome scanning; cellular stress; posttranscriptional regulation; MESSENGER-RNA TRANSLATION; AUG INITIATOR CODON; EUKARYOTIC TRANSLATION; MAMMALIAN-CELLS; GENE-EXPRESSION; REINITIATION; STRESS; GCN4; EFFICIENCY; NUCLEOTIDES;
D O I：
10.4161/bioe.27607
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.
引用
收藏
页码：186 / 192
页数：7
相关论文
共 35 条
  • [1] Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans
    Calvo, Sarah E.
    Pagliarini, David J.
    Mootha, Vamsi K.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (18) : 7507 - 7512
  • [2] FEREIRA JP, BIOENGINEERED, P20041
  • [3] Optimization of oncogene expression through intra-population competition
    Ferreira, Joshua P.
    Wang, Clifford L.
    [J]. BIOTECHNOLOGY JOURNAL, 2013, 8 (12) : 1476 - 1484
  • [4] Tuning gene expression with synthetic upstream open reading frames
    Ferreira, Joshua P.
    Overton, K. Wesley
    Wang, Clifford L.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (28) : 11284 - 11289
  • [5] Ferreira Joshua P, 2011, Syst Synth Biol, V5, P131, DOI 10.1007/s11693-011-9089-0
  • [6] Quantitative assessment of Ras over-expression via shotgun deployment of vectors utilizing synthetic promoters
    Ferreira, Joshua P.
    Lawhorn, Ingrid E. B.
    Peacock, Ryan W. S.
    Wang, Clifford L.
    [J]. INTEGRATIVE BIOLOGY, 2012, 4 (01) : 108 - 114
  • [7] REQUIREMENTS FOR INTERCISTRONIC DISTANCE AND LEVEL OF EUKARYOTIC INITIATION FACTOR-II ACTIVITY IN REINITIATION ON GCN4 MESSENGER-RNA VARY WITH THE DOWNSTREAM CISTRON
    GRANT, CM
    MILLER, PF
    HINNEBUSCH, AG
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) : 2616 - 2628
  • [8] EFFECT OF SEQUENCE CONTEXT AT STOP CODONS ON EFFICIENCY OF REINITIATION IN GCN4 TRANSLATIONAL CONTROL
    GRANT, CM
    HINNEBUSCH, AG
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (01) : 606 - 618
  • [9] TRANSLATIONAL ACTIVATION OF THE NON-AUG-INITIATED C-MYC-1 PROTEIN AT HIGH CELL DENSITIES DUE TO METHIONINE DEPRIVATION
    HANN, SR
    SLOANBROWN, K
    SPOTTS, GD
    [J]. GENES & DEVELOPMENT, 1992, 6 (07) : 1229 - 1240
  • [10] Regulated translation initiation controls stress-induced gene expression in mammalian cells
    Harding, HP
    Novoa, I
    Zhang, YH
    Zeng, HQ
    Wek, R
    Schapira, M
    Ron, D
    [J]. MOLECULAR CELL, 2000, 6 (05) : 1099 - 1108
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